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Details for Product No. ABIN967775

Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8) (AA 628-821) antibody

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Antigen
Synonyms AW261790, MGC81285, MGC146320
Epitope
»Alternatives AA 628-821
Reactivity
»Alternatives Mouse (Murine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Pubmed 4 references available
Catalog no. ABIN967775
Quantity 150 µg
Price
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Immunogen Mouse Eps8
Clone Ghr5
Isotype IgG1
Cross-Reactivity Human, Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Eps8
Background The p97 [Eps8] protein, a substrate for EGF-R tyrosine kinase, contains an SH3 domain, but lacks a functional SH2 domain. Antibodies to Eps8 recognize the 97 kDa protein and a less abundant 68 kDa protein. Both forms are tyrosine-phosphorylated following treatment of cells with EGF. It is likely that p68 [Eps8] is synthesized from an alternatively spliced mRNA since two major Eps8-specific mRNAs are detected by Northern analysis. Co-immunoprecipitation studies have demonstrated a physical association between the Eps8 protein and the EGF-R both in vivo and in vitro. For many EGF-R substrates, this interaction is mediated through an SH2 domain of the substrate. Since Eps8 lacks a well defined SH2 domain and a fusion protein containing the SH2-like region of Eps8 could not bind EGF-R, the mechanism of Eps8-EGF-R association remains unclear. Overexpression of Eps8 in fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF, suggesting that Eps8 has a role in the modulation of EGF-R function.
Molecular Weight 97 kDa
Comment

Related Products: ABIN967389, ABIN968550

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Xie, Cho, Calaycay et al.: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, 1992 (PubMed).

Fazioli, Minichiello, Matoska et al.: "Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals." in: The EMBO journal, Vol. 12, Issue 10, pp. 3799-808, 1993 (PubMed).

Miyamoto, Teramoto, Gutkind et al.: "Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: roles of integrin aggregation and occupancy of receptors." in: The Journal of cell biology, Vol. 135, Issue 6 Pt 1, pp. 1633-42, 1997 (PubMed).

Burke, Schooler, Wiley: "Regulation of epidermal growth factor receptor signaling by endocytosis and intracellular trafficking." in: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1897-910, 2001 (PubMed).

Alternatives for antigen "Epidermal Growth Factor Receptor Pathway Substrate 8 (EPS8)", type "Antibodies"
Hosts (27), (5), (1)
Reactivities (31), (18), (16), (13), (13), (13), (12), (1), (1), (1)
Applications (18), (13), (10), (10), (4), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1), (1), (1), (1)
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