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CD45RO (CD45RO) antibody
| Antigen | CD45RO (CD45RO) |
| Clonality | Monoclonal (UCHL1) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Immunohistochemistry (IHC)
|
 |
3 references available |
| Catalog no. | ABIN96778 |
| Quantity | 1ml (100ug/ml approximately) (Variants) |
| Price | 259.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | CD45RO |
| Immunogen | BALB/C mice were immunized with IL-2 dependent T cell line, CA1. |
| Format | Supernatant |
| Isotype | IgG2a kappa (Matching secondary antibodies) |
| Clone | UCHL1 |
| Specificity | This antibody reacts with a 180 kD glycoprotein of CD45 family, occurring on most thymocytes and activated T cells but only on the portion of the resting T cells. It reacts with most thymocytes, a subpopulation of resting cells within both the CD4 and CD8 subsets and mature activated T cells. |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cell membrane |
| Concentration | 100ug/ml approximately |
| Buffer | This antibody is supplied as purified immunoglobulin containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | CD Antigens |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Publications
| Publications |
Terry, Brown, Beverley: "The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45." in: Immunology, Vol. 64, Issue 2, pp. 331-6, 1988 (PubMed).
Hall, DArdenne, Butler et al.: "New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections." in: Journal of clinical pathology, Vol. 40, Issue 2, pp. 151-6, 1987 (PubMed). Magatti, De Munari, Vertua et al.: "Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 1, pp. 182-92, 2008 (PubMed). |
Alternatives
Alternatives for antigen "CD45RO (CD45RO)", type "Antibodies"
| Hosts | Mouse (67), Rabbit (20) |
| Reactivities | Human (85), Mouse (Murine) (19), Rat (Rattus) (19), Cow (Bovine) (3), Horse (Equine) (2), Pig (Porcine) (2), Chicken (1), Dog (Canine) (1), Rabbit (1) |
| Applications | Flow Cytometry (FACS) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Immunofluorescence (IF) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Immunoprecipitation (IP) (18), Immunohistochemistry (IHC) (11), Western Blotting (WB) (6), ELISA (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | FITC (10), Biotin (7), PE (7), APC (5), RPE (4), Cy5,PE (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), RBITC (1), RPE,Cy5 (1) |
