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CD45RO (CD45RO) antibody
| Antigen | CD45RO (CD45RO) |
| Clonality | Monoclonal (UCHL1) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|
 |
2 references available |
| Catalog no. | ABIN96779 |
| Quantity | 6ml (1 to 4 ug/ml) (Variants) |
| Price | 157.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | CD45RO |
| Immunogen | BALB/C mice were immunized with IL-2 dependent T cell line, CA1. |
| Format | Liquid (prediluted) |
| Isotype | IgG2a kappa (Matching secondary antibodies) |
| Clone | UCHL1 |
| Specificity | This antibody reacts with a 180 kD glycoprotein of CD45 family, occurring on most thymocytes and activated T cells but only on the portion of the resting T cells. It reacts with most thymocytes, a subpopulation of resting cells within both the CD4 and CD8 subsets and mature activated T cells. |
Application Details
| Application Notes | Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Overfixation in formalin should be avoided. Enzymatic predigestion can cause false positive staining of non T cell tumors. Positive Control: Tonsil. Cellular localization: Cell membrane |
| Concentration | 1 to 4 ug/ml |
| Buffer | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | CD Antigens |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Publications
| Publications |
Terry, Brown, Beverley: "The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45." in: Immunology, Vol. 64, Issue 2, pp. 331-6, 1988 (PubMed).
Hall, DArdenne, Butler et al.: "New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections." in: Journal of clinical pathology, Vol. 40, Issue 2, pp. 151-6, 1987 (PubMed). |
Alternatives
Alternatives for antigen "CD45RO (CD45RO)", type "Antibodies"
| Hosts | Mouse (67), Rabbit (20) |
| Reactivities | Human (85), Mouse (Murine) (19), Rat (Rattus) (19), Cow (Bovine) (3), Horse (Equine) (2), Pig (Porcine) (2), Chicken (1), Dog (Canine) (1), Rabbit (1) |
| Applications | Flow Cytometry (FACS) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Immunofluorescence (IF) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Immunoprecipitation (IP) (18), Immunohistochemistry (IHC) (12), Western Blotting (WB) (6), ELISA (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | FITC (10), Biotin (7), PE (7), APC (5), RPE (4), Cy5,PE (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), RBITC (1), RPE,Cy5 (1) |
