Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody

Antigen: Signal Transducer and Activator of Transcription 5A (STAT5A)

Synonyms: MGF, STAT5, AA959963, Stat5, STATA5, STAT5A/MGF, STAT5A

Binding Site: AA 451-649

Clonality: Monoclonal (89)

Host: Mouse

Reactivity: Sheep (Ovine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967811

Additional Information

Alternative name: Stat5

Immunogen: Sheep Stat5

Cross-Reactivity: Human, Dog (Canine), Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG2b

Clone: 89

Description: Stat5, also known as mammary gland factor (MGF) has been shown to play a critical role in the lactogenic hormone response and has been well characterized in mammary epithelial cells. The SH3 domain of MGF shows extensive homology with the SH2 domains of Stat1 and Stat2. This homology has led to its classification as a member of the Stat family. The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment and activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation.
Synonyms: MGF

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 92 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Receptors, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of Stat5 on human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Stat5 antibody.

Immunofluorescent staining of rabbit muscle with anti-Stat5 antibody.

Publications

Product: Gouilleux, Wakao, Mundt et al.: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994 (PubMed).

Kirken, Malabarba, Xu et al.: "Prolactin stimulates serine/tyrosine phosphorylation and formation of heterocomplexes of multiple Stat5 isoforms in Nb2 lymphocytes." in: The Journal of biological chemistry, Vol. 272, Issue 22, pp. 14098-103, 1997 (PubMed).

Sadowski, Choi, Le et al.: "Insulin Induction of SOCS-2 and SOCS-3 mRNA expression in C2C12 Skeletal Muscle Cells Is Mediated by Stat5*." in: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20703-10, 2001 (PubMed).

Chughtai, Schimchowitsch, Lebrun et al.: "Prolactin induces SHP-2 association with Stat5, nuclear translocation, and binding to the beta-casein gene promoter in mammary cells." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31107-14, 2002 (PubMed).

Buitenhuis, Baltus, Lammers et al.: "Signal transducer and activator of transcription 5a (STAT5a) is required for eosinophil differentiation of human cord blood-derived CD34+ cells." in: Blood, Vol. 101, Issue 1, pp. 134-42, 2002 (PubMed).