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RAP1 antibody (AA 1-184)

TERF2IP Reactivity: Human, Mouse, Rat, Chicken, Frog WB, IF, IP, IHC (f) Host: Mouse Monoclonal 3-Rap1 unconjugated
Catalog No. ABIN967815
  • Target See all RAP1 (TERF2IP) Antibodies
    RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))
    Binding Specificity
    • 10
    • 8
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-184
    Reactivity
    • 49
    • 13
    • 12
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Frog
    Host
    • 41
    • 6
    • 2
    Mouse
    Clonality
    • 42
    • 7
    Monoclonal
    Conjugate
    • 32
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RAP1 antibody is un-conjugated
    Application
    • 40
    • 20
    • 19
    • 9
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Cross-Reactivity
    Chicken, Frog, Mouse (Murine), Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Rap1 aa. 1-184
    Clone
    3-Rap1
    Isotype
    IgG1
    Top Product
    Discover our top product TERF2IP Primary Antibody
  • Comment

    Related Products: ABIN968537, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Larson, Chen, Kahn, Taylor, Fabre, Mortensen, Conley, Parise: "Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets." in: Blood, Vol. 101, Issue 4, pp. 1409-15, (2003) (PubMed).

    Wu, Lai, Mobley: "Nerve growth factor activates persistent Rap1 signaling in endosomes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 15, pp. 5406-16, (2001) (PubMed).

    Xing, Ge, Zeltser, Maskevitch, Mayer, Alexandropoulos: "c-Src signaling induced by the adapters Sin and Cas is mediated by Rap1 GTPase." in: Molecular and cellular biology, Vol. 20, Issue 19, pp. 7363-77, (2000) (PubMed).

    Okada, Pessin: "Insulin and epidermal growth factor stimulate a conformational change in Rap1 and dissociation of the CrkII-C3G complex." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28179-82, (1997) (PubMed).

    Yamamoto, Kaibuchi, Mizuno, Hiroyoshi, Shirataki, Takai: "Purification and characterization from bovine brain cytosol of proteins that regulate the GDP/GTP exchange reaction of smg p21s, ras p21-like GTP-binding proteins." in: The Journal of biological chemistry, Vol. 265, Issue 27, pp. 16626-34, (1990) (PubMed).

  • Target
    RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))
    Alternative Name
    Rap1 (TERF2IP Products)
    Synonyms
    DRIP5 antibody, RAP1 antibody, Rap1 antibody, cRAP1 antibody, TERF2 interacting protein antibody, telomeric repeat binding factor 2, interacting protein antibody, TERF2IP antibody, Terf2ip antibody
    Background
    Rap1 is a member of the large Ras superfamily of low molecular weight GTP/GDP binding proteins. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap1A and Rap1B are highly homologous proteins, differing in only 9 of their 184 amino acids. Overexpression of Rap1A (also known as Krev-1) causes reversion of the phenotype of a Ki-Ras-transformed cell line. In vitro, Rap1 can compete efficiently with p21ras for interaction with Ras-GAP. Though they appear to have similar activities, Rap1 and Ras differ in their cellular localization. Ras is found on the inner surface of the plasma membrane while Rap1 is associated with the Golgi. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    21 kDa
    Pathways
    Cell Division Cycle, Telomere Maintenance
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