BCL2-Like 1 (BCL2L1) (AA 18-233) antibody

Antigen: BCL2-Like 1 (BCL2L1)

Synonyms: BCLX, BCL2L, Bcl-X, bcl-xL, bcl-xS, BCL-XL/S, DKFZp781P2092, BclX, Bcl2l, bcl-x, Bcl-XL, Bcl-Xs, Bcl(X)L, MGC99998, MGC113803, Bclx, bcl-X, Bcl-xl, BCL2L1, Bcl-xL, BCL-XL, bclx, xR11, bcl2l, bclxl, bcl-XL, bcl-xs, MGC69340, bcl-xl/s, bcl-xl, Bcl-xS

Binding Site: AA 18-233

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967829

Additional Information

Alternative name: Bcl-x

Immunogen: Rat Bcl-xL

Cross-Reactivity: Human, Chicken, Dog (Canine), Mouse (Murine)

Format: Liquid

Isotype: IgG

Description: Bcl-x is related to the Bcl-2 protein and can function independently of Bcl-2 in regulating apoptosis (programmed cell death). The bcl-x transcript is found in a number of tissues, with the highest levels in the lymphoid and central nervous systems. Two distinct cDNA species, bcl-xL and bcl-xS, have been observed and appear to arise from alternate 5' splice sites located within the first coding exon of the bcl-x gene. Bcl-xL is composed of 233 amino acids and is similar in size and structure to Bcl-2. Bcl-xS lacks 63 amino acids corresponding to the region of Bcl-xL with the highest degree of homology to Bcl-2. Like Bcl-2, Bcl-xL inhibits cell death upon growth factor withdrawal in an IL-3-dependent cell line. However, Bcl-xS inhibits the ability of Bcl-2 to enhance cell survival in the absence of growth factors.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 26 kDa

Comments:

Related Products: ABIN968536, ABIN967390

Synonyms: BCLX, BCL2L, Bcl-X, bcl-xL, bcl-xS, BCL-XL/S, DKFZp781P2092, BclX, Bcl2l, bcl-x, Bcl-XL, Bcl-Xs, Bcl(X)L, MGC99998, MGC113803, Bclx, bcl-X, Bcl-xl, BCL2L1, Bcl-xL, BCL-XL, bclx, xR11, bcl2l, bclxl, bcl-XL, bcl-xs, MGC69340, bcl-xl/s, bcl-xl, Bcl-xS

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from antiserum by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Cancer

Restrictions: For Research Use only

Images

Western blot analysis of Bcl-x on human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Bcl-x antibody.

Immunofluorescent staining of human endothelial cells.

Publications

Product: Boise, González-García, Postema et al.: "bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death." in: Cell, Vol. 74, Issue 4, pp. 597-608, 1993 (PubMed).

Wen, Wang, McKay et al.: "Jak3 selectively regulates Bax and Bcl-2 expression to promote T-cell development." in: Molecular and cellular biology, Vol. 21, Issue 2, pp. 678-89, 2001 (PubMed).

Jiang, Clark: "Involvement of Bik, a proapoptotic member of the Bcl-2 family, in surface IgM-mediated B cell apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 10, pp. 6025-33, 2001 (PubMed).

Rogers, Song, Gramaglia et al.: "OX40 promotes Bcl-xL and Bcl-2 expression and is essential for long-term survival of CD4 T cells." in: Immunity, Vol. 15, Issue 3, pp. 445-55, 2001 (PubMed).

Tomicic, Thust, Kaina: "Ganciclovir-induced apoptosis in HSV-1 thymidine kinase expressing cells: critical role of DNA breaks, Bcl-2 decline and caspase-9 activation." in: Oncogene, Vol. 21, Issue 14, pp. 2141-53, 2002 (PubMed).