JAK1 antibody (Janus Kinase 1) (AA 551-766)

Details for Product anti-JAK1 Antibody No. ABIN967842, Supplier: Log in to see
Antigen
  • JNK
  • JNK1
  • JAK1
  • jak1
  • JAK1A
  • JAK1B
  • JTK3
  • AA960307
  • C130039L05Rik
  • wu:fb93e10
  • Janus kinase 1
  • mitogen-activated protein kinase 8
  • Tyrosine-protein kinase Jak1
  • JAK1
  • MAPK8
  • jak1
  • Jak1
Alternatives
anti-Human JAK1 antibody for Western Blotting
Epitope
AA 551-766
30
21
18
16
15
15
7
7
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
138
111
94
5
5
4
4
1
Host
Mouse
117
24
10
1
Clonality (Clone)
Monoclonal ()
Conjugate
This JAK1 antibody is un-conjugated
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
91
65
48
25
13
13
11
10
7
4
2
2
1
Options
Supplier
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Supplier Product No.
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Immunogen Human JAK1 aa. 551-766
Clone 73-JAK1
Isotype IgG1, kappa
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken, Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name JAK1 (JAK1 Antibody Abstract)
Background The JAK family of receptor-associated protein kinases is directly involved in interferon (IFN) response pathways. The JAK family contains at least three members: JAK1, JAK2, and Tyk2. Each protein is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among family members. In several human and murine cell lines, JAK1 is rapidly tyrosine phosphorylated in response to IFN-alpha and IFN-gamma. JAK1 is required for the phosphorylation of the transcription factor Stat1 (p91), in response to IFNs-alpha or IFN-gamma. JAK1 is also necessary for the efficient phophorylation of Stat2 (p113) in response to IFN-alpha and for the phosphorylation of Tyk2 or JAK2 in response to IFNs-alpha or IFN-gamma, respectively.
Molecular Weight 130 kDa
Research Area Signaling, Cytokines, Receptors, Kinases/Phosphatases
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded protein response
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Janus Kinase 1 (JAK1) (AA 551-766) antibody (ABIN967842) Western blot analysis of JAK1 on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, la...
Immunofluorescence (IF) image for anti-Janus Kinase 1 (JAK1) (AA 551-766) antibody (ABIN967842) Immunofluorescent staining of Human Endothelial cells.
 image for anti-Janus Kinase 1 (JAK1) (AA 551-766) antibody (ABIN967842) anti-Janus Kinase 1 (JAK1) (AA 551-766) antibody (Image 3)
Product cited in: Kopantzev, Heller, Swaminathan, Rudikoff: "IL-6 mediated activation of STAT3 bypasses Janus kinases in terminally differentiated B lineage cells." in: Oncogene, Vol. 21, Issue 44, pp. 6791-800, 2002 (PubMed).

Blesofsky, Mowen, Arduini, Baker, Murphy, Bowtell, David: "Regulation of STAT protein synthesis by c-Cbl." in: Oncogene, Vol. 20, Issue 50, pp. 7326-33, 2001 (PubMed).

Kawazoe, Naka, Fujimoto, Kohzaki, Morita, Narazaki, Okumura, Saitoh, Nakagawa, Uchiyama, Akira, Kishimoto: "Signal transducer and activator of transcription (STAT)-induced STAT inhibitor 1 (SSI-1)/suppressor of cytokine signaling 1 (SOCS1) inhibits insulin signal transduction pathway through modulating insulin receptor substrate 1 (IRS-1) phosphorylation." in: The Journal of experimental medicine, Vol. 193, Issue 2, pp. 263-9, 2001 (PubMed).

Müller, Briscoe, Laxton, Guschin, Ziemiecki, Silvennoinen, Harpur, Barbieri, Witthuhn, Schindler: "The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction." in: Nature, Vol. 366, Issue 6451, pp. 129-35, 1993 (PubMed).

Background publications Nicholson, Oates, Harpur, Ziemiecki, Wilks, Layton: "Tyrosine kinase JAK1 is associated with the granulocyte-colony-stimulating factor receptor and both become tyrosine-phosphorylated after receptor activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 8, pp. 2985-8, 1994 (PubMed).

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