Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2)

Synonyms: MEK2, MKK2, MAPKK2, PRKMK2, FLJ26075

Binding Site: AA 1-110

Clonality: Monoclonal (96)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN967846

Additional Information

Alternative name: MEK2

Immunogen: Rat MEK2

Cross-Reactivity: Mouse (Murine), Chicken, Dog (Canine), Frog, Human

Format: Liquid

Isotype: IgG2a

Clone: 96

Description: MEK2 (MAP Kinase Kinase 2 or ERK Kinase), a 46 kDa protein kinase, phosphorylates MAP kinases (ERKs) at tyrosine and threonine residues. This phosphorylation results in activation of the MAP kinases. MEK2 is seven amino acids larger and shares 81% identity with MEK1. In cultured cells, MEK2 is activated by serum. In vitro, v-Raf phosphorylates and activates MEK2. It is thought that all of these activated protein kinases are downstream of the Ras signal transduction pathway and represent an integral part of the Ras mitogenic signal. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase Kinase 2, ERK Kinase

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 46 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cancer, Tyrosine Kinases, Kinases/Phosphatases

Restrictions: For Research Use only

Images

Western blot analysis of MEK2 on a RSV-3T3 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of the anti- MEK2 antibody.

Immunofluorescence staining of human fibroblasts.

Publications

Product: Hattori, Fukuda, Yamashita et al.: "Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system." in: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20346-51, 1992 (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Wu, Harrison, Dent et al.: "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4539-48, 1993 (PubMed).

Downey, Butler, Tapper et al.: "Importance of MEK in neutrophil microbicidal responsiveness." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 434-43, 1998 (PubMed).

Tworkowski, Salghetti, Tansey: "Stable and unstable pools of Myc protein exist in human cells." in: Oncogene, Vol. 21, Issue 55, pp. 8515-20, 2002 (PubMed).