CDKN1B antibody (AA 1-197)

Catalog No. ABIN967851

Product Details anti-CDKN1B Antibody

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Binding Specificity: AA 1-197

Reactivity: Human, Mouse, Rat, Dog, Chicken, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus), Chicken, Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Kip1 aa. 1-197

Clone: 57-Kip1-p27

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CDKN1B antibody (ABIN967851)

Barnouin, Dubuisson, Child, Fernandez de Mattos, Glassford, Medema, Mann, Lam: "H2O2 induces a transient multi-phase cell cycle arrest in mouse fibroblasts through modulating cyclin D and p21Cip1 expression." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13761-70, (2002) (PubMed).

Williamson, Dadmanesh, Koeffler: "BRCA1 transactivates the cyclin-dependent kinase inhibitor p27(Kip1)." in: Oncogene, Vol. 21, Issue 20, pp. 3199-206, (2002) (PubMed).

Frey, Clark, Leontieva, Uronis, Black, Black: "Protein kinase C signaling mediates a program of cell cycle withdrawal in the intestinal epithelium." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 763-78, (2000) (PubMed).

Polyak, Kato, Solomon, Sherr, Massague, Roberts, Koff: "p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest." in: Genes & development, Vol. 8, Issue 1, pp. 9-22, (1994) (PubMed).

Polyak, Lee, Erdjument-Bromage, Koff, Roberts, Tempst, Massagué: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, (1994) (PubMed).

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: p27 Kip1 (CDKN1B Products)

Synonyms: CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, cdkn1b antibody, sb:cb611 antibody, wu:fb63g10 antibody, wu:fb64g10 antibody, wu:fe18e03 antibody, CDKN1B antibody, Kip1 antibody, p27 antibody, FAM14D antibody, ISG12 antibody, ISG12A antibody, P27 antibody, Cdki1b antibody, AA408329 antibody, AI843786 antibody, p27Kip1 antibody, cdkn1bl antibody, cyclin dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Bb antibody, interferon alpha inducible protein 27 antibody, cyclin-dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Ba antibody, CDKN1B antibody, cdkn1bb antibody, IFI27 antibody, Cdkn1b antibody, cdkn1ba antibody

Background: Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in TGFbeta-induced G1 phase arrest and cell-cell contact. In vitro , p27 [Kip1] binds tightly to Cyclin D-Cdk4, Cyclin E-Cdk2, and Cyclin A-Cdk2 complexes and inhibits their activity. In normal cells, Kip1 is sequestered and its activity gradually decreases as cells reach S phase. The addition of TGF-beta in early G1 blocks this decline in activity, by preventing the sequestration of Kip1. TGF-beta also reduces the levels of Cdk4. Kip1 preferentially binds Cyclin D-Cdk4, but the lower levels of Cdk4 in TGF-beta treated cells allow Kip1 to be available for binding to Cyclin E-Cdk2 and Cyclin A-Cdk2. Kip1 is structurally related to Cip1/WAF1, having a similar 60 amino acid sequence in the N-terminal region. A 52 amino acid Kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro. This antibody is routinely tested by western blot analysis.

Molecular Weight: 27 kDa

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy