p130 antibody (AA 26-367)

Catalog No. ABIN967864

Product Details anti-p130 Antibody

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Binding Specificity: AA 26-367

Reactivity: Human, Rat, Mouse, Chicken, Blow Fly

Host: Mouse

Clonality: Monoclonal

Conjugate: This p130 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken, Fruit Fly (Drosophila melanogaster)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rb2 aa. 26-367

Clone: 10-Rb2

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-p130 antibody (ABIN967864)

Fusaro, Wang, Chellappan: "Differential regulation of Rb family proteins and prohibitin during camptothecin-induced apoptosis." in: Oncogene, Vol. 21, Issue 29, pp. 4539-48, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Laplantine, Rossi, Sahni, Basilico, Cobrinik: "FGF signaling targets the pRb-related p107 and p130 proteins to induce chondrocyte growth arrest." in: The Journal of cell biology, Vol. 158, Issue 4, pp. 741-50, (2002) (PubMed).

Mayol, Graña, Baldi, Sang, Hu, Giordano: "Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain." in: Oncogene, Vol. 8, Issue 9, pp. 2561-6, (1993) (PubMed).

Yeung, Bell, Testa, Mayol, Baldi, Graña, Klinga-Levan, Knudson, Giordano: "The retinoblastoma-related gene, RB2, maps to human chromosome 16q12 and rat chromosome 19." in: Oncogene, Vol. 8, Issue 12, pp. 3465-8, (1993) (PubMed).

Target Details for p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Alternative Name: Rb2 (RBL2 Products)

Synonyms: RBL2 antibody, RBR2 antibody, RRB2a antibody, PRB2 antibody, RBR-2 antibody, Rb2 antibody, p130 antibody, P130 antibody, retinoblastoma-like 2 (p130) antibody, RB transcriptional corepressor like 2 antibody, related to retinoblastoma 2 antibody, RBL2 antibody, LOC541652 antibody, Rbl2 antibody

Background: The pRb2 protein shows a high degree of identity with pRb and the related p107 protein. Both pRb and p107 bind to the adenovirus E1A, SV40 large T antigen, and papillomavirus E7 viral proteins. This binding initiates the release of transcription factors which are required for the expression of cell cycle-regulated genes. pRb, pRb2, and p107 interact with a conserved motif in these three viral proteins. In the E1A protein, this area is known as transforming domain 2, which is required for growth activation. The E1A-binding domain in pRb and p107 is a conserved motif known as the pocket region which consists of conserved A and B regions separated by non-conserved spacers of different sizes in pRb and p107. It is the pocket regions of pRb and p107 that associate with the E2F transcription factor. pRb2 also contains the conserved pocket region suggesting that it has functional similarities to pRb and p107. This antibody is routinely tested by western blot analysis.

Molecular Weight: 130 kDa

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases