CD45 antibody (AA 535-730)

Catalog No. ABIN967867

Product Details anti-CD45 Antibody

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Binding Specificity: AA 535-730

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD45 aa. 535-730 (alternatively spliced)

Clone: 69-CD45

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CD45 antibody (ABIN967867)

Wu, Fu, Shen: "SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription." in: Molecular and cellular biology, Vol. 22, Issue 8, pp. 2673-86, (2002) (PubMed).

Wang, Liang, Esselman: "Regulation of the calcium/NF-AT T cell activation pathway by the D2 domain of CD45." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 5, pp. 2557-64, (2000) (PubMed).

Phipps, Yousefi, Branch: "Increased enzymatic activity of the T-cell antigen receptor-associated fyn protein tyrosine kinase in asymptomatic patients infected with the human immunodeficiency virus." in: Blood, Vol. 90, Issue 9, pp. 3603-12, (1997) (PubMed).

Biffen, McMichael-Phillips, Larson, Venkitaraman, Alexander: "The CD45 tyrosine phosphatase regulates specific pools of antigen receptor-associated p59fyn and CD4-associated p56lck tyrosine in human T-cells." in: The EMBO journal, Vol. 13, Issue 8, pp. 1920-9, (1994) (PubMed).

Sieh, Bolen, Weiss: "CD45 specifically modulates binding of Lck to a phosphopeptide encompassing the negative regulatory tyrosine of Lck." in: The EMBO journal, Vol. 12, Issue 1, pp. 315-21, (1993) (PubMed).

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45 (Ly-5) (PTPRC Products)

Synonyms: PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

Background: CD45 (also known as leukocyte common antigen, GP180, or T200) is found on all hematopoietic cells except those of red cell origin. The N-terminal domain is a large glycosylated extracellular region of variable length (390-542 amino acids) derived by the alternative splicing of at least three exons (4, 5, or 6). This variation accounts for the different isoforms of CD45 (180-220 kDa). The remainder of the molecule includes a short transmembrane region followed by a large highly conserved cytoplasmic domain of 702 residues. The CD45 cytoplasmic domain possesses an intrinsic protein tyrosine phosphatase (PTPase) activity. This region contains two PTPase domains (domains I and II) which share 40% homology. Both domains are required for optimal PTPase activity and show significant homology with other receptor-like and cytoplasmic PTPases. CD45 plays a critical role in antigen receptor-induced responses of T and B cells. In B cells, CD45 can exist as part of a larger multisubunit complex composed of several tyrosine phosphorylated proteins, including the Src-family PTK, Lyn, or it can exist as the membrane immunoglobulin-associated MB-1/B29 heterodimer. In T cells, CD45 associates with and dephosphorylates both lck and fyn. In addition, CD45 can associate with membrane proteins involved in T cell activation, such as CD2, Thy-1, TCR, and CD26.
Synonyms: Leukocyte Common Antigen, GP180, T200

Molecular Weight: 180-220 kDa

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling