Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-241) antibody
|Synonyms||CaMK-GR, MGC36771, CAMK4, CaMKIV, AI666733, CaMKIV/Gr, D18Bwg0362e, A430110E23Rik, Ccdpk, RATCCDPK, camk4-A, wu:fj42a03, zgc:110275|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
|5 references available|
|Price||Product not available in this region.|
|Alternative name||CaM Kinase IV|
|Immunogen||Human CaM Kinase IV|
|Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Description||CaM-kinase IV (Ca2+/calmodulin-dependent protein kinase IV, also described as CaM-kinase Gr) is activated through the binding of Ca2+/CaM and by phosphorylation. This kinase has high sequence homologies with the catalytic and regulatory domains of CaM-kinase II. CaM-kinase IV is a monomer which is highly expressed in cerebellum, thymus, and testis. Its subcellular distrubution includes localization in both the synaptic regions of the molecular layer of the cerebellum, and the nuclei of cerebellar granule cells. CaM-kinase IV has an autoinhibitory domain within residues 305-321 that can suppress kinase activity in the absence of Ca2+/CaM. This type of domain is common in many other CaM-dependent kinases and phosphatases. CaM-kinase IV does not appear to be significantly activated by autophosphorylation, but it can be activated approximately 5-10-fold when phosphorylated by CaM-kinase IV kinase.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||60 kDa|
Related Products: ABIN968537, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
Ohmstede, Bland, Merrill et al.: "Relationship of genes encoding Ca2+/calmodulin-dependent protein kinase Gr and calspermin: a gene within a gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 13, pp. 5784-8, 1991 (PubMed).
Passier, Zeng, Frey et al.: "CaM kinase signaling induces cardiac hypertrophy and activates the MEF2 transcription factor in vivo." in: The Journal of clinical investigation, Vol. 105, Issue 10, pp. 1395-406, 2000 (PubMed).
Slee, Adrain, Martin: "Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7320-6, 2001 (PubMed).
Wayman, Walters, Kolibaba et al.: "CaM kinase IV regulates lineage commitment and survival of erythroid progenitors in a non-cell-autonomous manner." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 811-24, 2000 (PubMed).
Wei, Qiu, Liauw et al.: "Calcium calmodulin-dependent protein kinase IV is required for fear memory." in: Nature neuroscience, Vol. 5, Issue 6, pp. 573-9, 2002 (PubMed).