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RAB5 antibody (AA 1-215)

RAB5A Reactivity: Human, Mouse, Rat WB, IHC, IF, IP Host: Mouse Monoclonal 15-Rab5 unconjugated
Catalog No. ABIN967882
  • Target See all RAB5 (RAB5A) Antibodies
    RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))
    Binding Specificity
    • 17
    • 15
    • 15
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-215
    Reactivity
    • 99
    • 64
    • 44
    • 12
    • 11
    • 11
    • 10
    • 10
    • 9
    • 9
    • 8
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 101
    • 10
    • 4
    • 2
    Mouse
    Clonality
    • 96
    • 21
    Monoclonal
    Conjugate
    • 54
    • 8
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RAB5 antibody is un-conjugated
    Application
    • 87
    • 40
    • 37
    • 33
    • 26
    • 19
    • 16
    • 14
    • 13
    • 8
    • 5
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Cross-Reactivity
    Rat (Rattus), Mouse (Murine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Rab5 aa. 1-215
    Clone
    15-Rab5
    Isotype
    IgG1
    Top Product
    Discover our top product RAB5A Primary Antibody
  • Comment

    Related Products: ABIN968536, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Huang, Imamura, Olefsky: "Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 23, pp. 13084-9, (2001) (PubMed).

    Wang, Hu, Zimmermann, Kilimann: "Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal alpha -helix motifs." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32480-8, (2001) (PubMed).

    Pinkoski, Hobman, Heibein, Tomaselli, Li, Seth, Froelich, Bleackley: "Entry and trafficking of granzyme B in target cells during granzyme B-perforin-mediated apoptosis." in: Blood, Vol. 92, Issue 3, pp. 1044-54, (1998) (PubMed).

    Li, Stahl: "Structure-function relationship of the small GTPase rab5." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 24475-80, (1993) (PubMed).

    Sanford, Pan, Wessling-Resnick: "Prenylation of Rab5 is dependent on guanine nucleotide binding." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 23773-6, (1993) (PubMed).

  • Target
    RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))
    Alternative Name
    Rab5 (RAB5A Products)
    Synonyms
    RAB5 antibody, 2410015H04Rik antibody, AI663973 antibody, AU021172 antibody, nnyRab5a antibody, DDBDRAFT_0168594 antibody, DDBDRAFT_0229401 antibody, DDB_0168594 antibody, DDB_0229401 antibody, rab5 antibody, RAB5A antibody, Rab5a antibody, rab5al antibody, zgc:56644 antibody, fj38g08 antibody, hm:zehn1144 antibody, rab5a antibody, wu:fj38g08 antibody, RAB5A, member RAS oncogene family antibody, rab5A protein antibody, Rab5a, GTPase antibody, predicted protein antibody, Rab GTPase antibody, RAB5A, member RAS oncogene family L homeolog antibody, RAB5A, member RAS oncogene family, b antibody, RAB5A, member RAS oncogene family, a antibody, RAB5A antibody, Rab5a antibody, rab5A antibody, rab5a antibody, rab5a.L antibody, rab5ab antibody, rab5aa antibody
    Background
    Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post-translationally modified by geranylgeranylation, which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 is involved in regulation of early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and organelle membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 insertion into membranes is a multistep process in which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol.
    Molecular Weight
    25 kDA
    Pathways
    Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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