Interferon Regulatory Factor 9 (IRF9) (AA 126-351) antibody

Antigen: Interferon Regulatory Factor 9 (IRF9)

Synonyms: p48, IRF-9, ISGF3, ISGF3G

Binding Site: AA 126-351

Clonality: Monoclonal (6)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN967883

Additional Information

Alternative name: ISGF3 gamma

Immunogen: Human ISGF3gamma (p48)

Format: Liquid

Isotype: IgG1

Clone: 6

Description: ISGF3gamma is a nuclear DNA-binding protein. ISGF3 (Interferon Stimulated Gene Factor-3) is the primary transcription activator induced by the binding of interferon alpha (IFNalpha) to cell surface receptors. The functional ISGF3 is a complex of four polypeptides of 113 kDa (Stat2), 91 kDa (Stat1alpha), 84 kDa (Stat1beta), and 48 kDa. The first three polypeptides (also known as ISGF3gamma) each contain SH2 and SH3 domains. In response to either IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 binding their respective receptors, the cytoplasmic ISGF3alpha subunits are tyrosine-phosphorylated and translocated to the nucleus where they form an active complex with the 48 kDa protein (ISGF3gamma). This complex is responsible for modulating the transcription of interferon-stimulated genes (ISGs). Activated ISGF3alpha stabilizes ISGF3gamma-DNA interactions. This results in a ternary protein-DNA complex with a 25-fold greater stability than ISGF3gamma-DNA. Thus, growth factors and cytokines activate a common signal transduction pathway that leads to the phosphorylation and nuclear translocation of a group of latent cytoplasmic transcription factors.
Synonyms: Interferon Stimulated Gene Factor-3

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 48 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of ISGF3gamma on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human ISGF3gamma antibody.

Immunofluorescence staining of human fibroblasts.

anti-Interferon Regulatory Factor 9 (IRF9) (AA 126-351) antibody (Image 3)

Publications

Product: Veals, Schindler, Leonard et al.: "Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of DNA-binding proteins." in: Molecular and cellular biology, Vol. 12, Issue 8, pp. 3315-24, 1992 (PubMed).

Köster, Hauser: "Dynamic redistribution of STAT1 protein in IFN signaling visualized by GFP fusion proteins." in: European journal of biochemistry / FEBS, Vol. 260, Issue 1, pp. 137-44, 1999 (PubMed).

Dumler, Kopmann, Wagner et al.: "Urokinase induces activation and formation of Stat4 and Stat1-Stat2 complexes in human vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24059-65, 1999 (PubMed).