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IRF9 antibody (Interferon Regulatory Factor 9) (AA 126-351)

Details for Product anti-IRF9 Antibody No. ABIN967883, Supplier: Login to see
Antigen
  • IRF-9
  • ISGF3
  • ISGF3G
  • p48
  • zgc:92097
  • Irf-9
  • Isgf3g
Alternatives
anti-Human IRF9 antibody for Immunohistochemistry
Epitope
AA 126-351
13
11
9
6
5
3
3
3
2
1
1
Reactivity
Human
56
21
5
1
1
1
1
Host
Mouse
52
12
3
Clonality (Clone)
Monoclonal ()
Conjugate
This IRF9 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
49
24
24
16
11
11
10
3
2
Supplier
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Immunogen Human ISGF3gamma (p48) aa.126-351
Clone 6-ISGF3gamma
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ISGF3 gamma (IRF9 Antibody Abstract)
Background ISGF3gamma is a nuclear DNA-binding protein. ISGF3 (Interferon Stimulated Gene Factor-3) is the primary transcription activator induced by the binding of interferon alpha (IFNalpha) to cell surface receptors. The functional ISGF3 is a complex of four polypeptides of 113 kDa (Stat2), 91 kDa (Stat1alpha), 84 kDa (Stat1beta), and 48 kDa. The first three polypeptides (also known as ISGF3gamma) each contain SH2 and SH3 domains. In response to either IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 binding their respective receptors, the cytoplasmic ISGF3alpha subunits are tyrosine-phosphorylated and translocated to the nucleus where they form an active complex with the 48 kDa protein (ISGF3gamma). This complex is responsible for modulating the transcription of interferon-stimulated genes (ISGs). Activated ISGF3alpha stabilizes ISGF3gamma-DNA interactions. This results in a ternary protein-DNA complex with a 25-fold greater stability than ISGF3gamma-DNA. Thus, growth factors and cytokines activate a common signal transduction pathway that leads to the phosphorylation and nuclear translocation of a group of latent cytoplasmic transcription factors.
Synonyms: Interferon Stimulated Gene Factor-3
Molecular Weight 48 kDa
Pathways JAK-STAT Signaling, Interferon-gamma Pathway
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-IRF9 antibody (Interferon Regulatory Factor 9) (AA 126-351) (ABIN967883) Western blot analysis of ISGF3gamma on a human endothelial cell lysate. Lane 1: 1:250...
Immunofluorescence (IF) image for anti-IRF9 antibody (Interferon Regulatory Factor 9) (AA 126-351) (ABIN967883) Immunofluorescence staining of human fibroblasts.
 image for anti-IRF9 antibody (Interferon Regulatory Factor 9) (AA 126-351) (ABIN967883) anti-Interferon Regulatory Factor 9 (IRF9) (AA 126-351) antibody (Image 3)
Western Blotting (WB) image for anti-IRF9 antibody (Interferon Regulatory Factor 9) (AA 126-351) (ABIN967883) anti-Interferon Regulatory Factor 9 (IRF9) (AA 126-351) antibody (Image 4)
Product cited in: Dumler, Kopmann, Wagner et al.: "Urokinase induces activation and formation of Stat4 and Stat1-Stat2 complexes in human vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24059-65, 1999 (PubMed).

Köster, Hauser: "Dynamic redistribution of STAT1 protein in IFN signaling visualized by GFP fusion proteins." in: European journal of biochemistry / FEBS, Vol. 260, Issue 1, pp. 137-44, 1999 (PubMed).

Veals, Schindler, Leonard et al.: "Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of DNA-binding proteins." in: Molecular and cellular biology, Vol. 12, Issue 8, pp. 3315-24, 1992 (PubMed).