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DAP Kinase 1 antibody (Death-Associated Protein Kinase 1) (AA 694-947)

Details for Product anti-DAPK1 Antibody No. ABIN967884, Supplier: Login to see
Antigen
  • DAPK1
  • MGC81366
  • si:ch211-66i11.1
  • wu:fj09c03
  • dapk
  • MGC83745
  • 2310039H24Rik
  • 2810425C21Rik
  • AI642003
  • D13Ucla1
  • DAP-Kinase
  • DAPK
Alternatives
anti-Human DAP Kinase 1 antibody for Western Blotting
Epitope
AA 694-947
20
20
15
13
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
83
33
18
8
1
Host
Mouse
85
7
Clonality (Clone)
Monoclonal ()
Conjugate
This DAP Kinase 1 antibody is un-conjugated
5
5
5
4
4
4
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
57
46
29
13
13
12
2
2
2
2
1
1
1
Supplier
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Immunogen Human DAP Kinase aa. 694-947
Clone 17-DAP Kinase
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name DAP Kinase (DAPK1 Antibody Abstract)
Background Chronic exposure to extracellular signals such as interferons induce the inhibition of cell proliferation followed by cell death. The gene for DAP Kinase (Death Associated Protein Kinase) was identified using a novel approach named Technical Knockout. Briefly, Hela cells were transfected with an antisense cDNA expression library, then exposed to Interferon-gamma. The surviving cells, with their antisense cDNAs, were rescued and the protecting genes isolated. The DAP Kinase gene encodes a protein of 1423 amino acids, a molecular weight of 160kDa, a kinase domain at its amino terminal region, ankyrin repeats in the middle region, and a death domain at the extreme C-terminus. DAP Kinase phosphorylates at Ser/Thr residues in a Ca2+/Calmodulin-dependent fashion. It has been demonstrated that Ca2+/Calmodulin binds directly to DAP Kinase at its amino terminal region. In addition, immunostaining studies localized DAP Kinase in association with the actin filaments where it may phosphorylate myosin light chain. Thus, this novel cytoskeletal and Ca2+/Calmodulin-dependent protein kinase plays a role in interferon-gamma-induced cell death. This antibody is routinely tested by western blot analysis.
Molecular Weight 160 kDa
Pathways MAPK Signaling, Interferon-gamma Pathway
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-DAP Kinase 1 antibody (Death-Associated Protein Kinase 1) (AA 694-947) (ABIN967884) Western blot analysis of DAP Kinase on a SKN (human neuroblastoma) lysate. Lane 1: 1:...
 image for anti-DAP Kinase 1 antibody (Death-Associated Protein Kinase 1) (AA 694-947) (ABIN967884) Immunoflourescence staining of human fibroblasts.
Product cited in: Cohen, Feinstein, Kimchi: "DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity." in: The EMBO journal, Vol. 16, Issue 5, pp. 998-1008, 1997 (PubMed).

Background publications Deiss, Feinstein, Berissi et al.: "Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death." in: Genes & development, Vol. 9, Issue 1, pp. 15-30, 1995 (PubMed).