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ENOS antibody (Nitric Oxide Synthase 3 (Endothelial Cell)) (AA 1025-1203)

Details for Product anti-NOS3 Antibody No. ABIN967886, Supplier: Log in to see
Antigen
  • NOS3
  • ECNOS
  • eNOS
  • NOS
  • EC-NOS
  • NOSIII
  • cNOS
  • 2310065A03Rik
  • Nos-3
  • ecNOS
  • eNos
  • ENOS
Alternatives
anti-Human ENOS antibody for Enzyme Immunoassay
Epitope
AA 1025-1203
51
24
24
21
19
18
15
14
14
13
13
13
9
9
9
9
6
6
5
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5
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3
3
2
2
2
2
2
2
2
2
2
1
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1
1
1
1
1
1
1
1
1
1
1
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1
1
1
1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
375
207
193
31
31
11
11
9
2
2
2
1
1
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Host
Mouse
408
38
Clonality (Clone)
Monoclonal ()
Conjugate
This ENOS antibody is un-conjugated
28
25
17
8
7
7
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6
6
6
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6
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
282
167
68
67
57
53
24
23
22
19
11
7
5
2
2
1
1
Supplier
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Immunogen Human eNOS aa. 1025-1203
Clone 3-eNOS-NOS Type III
Isotype IgG1, kappa
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name eNOS/NOS Type III (NOS3 Antibody Abstract)
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca[2+] levels and enhance calmodulin binding. Neuronal NOS (mNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and both are regulated in a similar manner. The human forms exhibit 52% amino acid identity. However, they are distinct gene products of about 155 kDa (mNOS) and 140 kDA (eNOS). The eNOS gene was cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC). eNOS protein has a unique N-myristylation consensus sequence that may explain its membrane localization.
Molecular Weight 140 kDa
Pathways ACE Inhibitor Pathway
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-ENOS antibody (Nitric Oxide Synthase 3 (Endothelial Cell)) (AA 1025-1203) (ABIN967886) Western blot analysis of eNOS/NOS Type III on human endothelial cell lysate. Lane 1: ...
Immunofluorescence (IF) image for anti-ENOS antibody (Nitric Oxide Synthase 3 (Endothelial Cell)) (AA 1025-1203) (ABIN967886) Immunofluorescent staining of Endothelial Cells
Product cited in: Kincer, Uittenbogaard, Dressman et al.: "Hypercholesterolemia promotes a CD36-dependent and endothelial nitric-oxide synthase-mediated vascular dysfunction." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23525-33, 2002 (PubMed).

Thomas, Chen, Keaney: "Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6017-24, 2002 (PubMed).

Cao, Yao, McCabe et al.: "Direct interaction between endothelial nitric-oxide synthase and dynamin-2. Implications for nitric-oxide synthase function." in: The Journal of biological chemistry, Vol. 276, Issue 17, pp. 14249-56, 2001 (PubMed).

Laufs, Endres, Stagliano et al.: "Neuroprotection mediated by changes in the endothelial actin cytoskeleton." in: The Journal of clinical investigation, Vol. 106, Issue 1, pp. 15-24, 2000 (PubMed).

Zhao, Dugas, Mathiot et al.: "B-cell chronic lymphocytic leukemia cells express a functional inducible nitric oxide synthase displaying anti-apoptotic activity." in: Blood, Vol. 92, Issue 3, pp. 1031-43, 1998 (PubMed).