Nitric Oxide Synthase 1 (Neuronal) (NOS1) antibody

Antigen: Nitric Oxide Synthase 1 (Neuronal) (NOS1)

Synonyms: NOS, nNOS, IHPS1, INOS, NOS2A, HEP-NOS, NO, bNOS, NOS-I, Nos-1, 2310005C01Rik, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, iNos, nos, NOS2, NOS1, nos2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Mouse (Murine), Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967897

Additional Information

Alternative name: nNOS/NOS Type I

Format: Liquid

Isotype: Ig

Description: Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS or bNOS) and endothelial NOS (ECNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155 kDa and 140 kDa, respectively, and the human forms show 52% amino acid identity. Neuronal NOS and induced macrophage NOS (iNOS) share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. Neuronal NOS, a cytosolic protein present mainly in neural tissues, has been purified and characterized from rat cerebellum. The NO synthesized by this enzyme acts as a neurotransmitter. ECNOS has been cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC) and has a unique N-myristylation consensus sequence that may explain its membrane localization. This polyclonal antibody was generated using human nNOS aa. 1095-1289 as immunogen.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 155 kDa

Comments:

Related Products: ABIN967390

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of nNOS/NOS Type I on a rat whole brain lysate using 2 µg/mL of the polyclonal rabbit anti-nNOS/NOS Type I antibody.

Immunofluorescence staining of rat neurons.

Publications

Product: Bredt, Hwang, Glatt et al.: "Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase." in: Nature, Vol. 351, Issue 6329, pp. 714-8, 1991 (PubMed).

Sciorati, Rovere, Ferrarini et al.: "Autocrine nitric oxide modulates CD95-induced apoptosis in gammadelta T lymphocytes." in: The Journal of biological chemistry, Vol. 272, Issue 37, pp. 23211-5, 1997 (PubMed).

Coers, Timens, Kempinga et al.: "Specificity of antibodies to nitric oxide synthase isoforms in human, guinea pig, rat, and mouse tissues." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 46, Issue 12, pp. 1385-92, 1999 (PubMed).

Bender, Demady, Osawa: "Ubiquitination of neuronal nitric-oxide synthase in vitro and in vivo." in: The Journal of biological chemistry, Vol. 275, Issue 23, pp. 17407-11, 2000 (PubMed).

Billecke, Bender, Kanelakis et al.: "hsp90 is required for heme binding and activation of apo-neuronal nitric-oxide synthase: geldanamycin-mediated oxidant generation is unrelated to any action of hsp90." in: The Journal of biological chemistry, Vol. 277, Issue 23, pp. 20504-9, 2002 (PubMed).