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Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 1-219)

Details for Product anti-CASP3 Antibody No. ABIN967902, Supplier: Log in to see
Antigen
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
Alternatives
anti-Human Caspase 3 antibody for Western Blotting
Epitope
AA 1-219
37
31
30
30
19
19
18
17
14
13
13
12
12
10
9
8
8
6
6
6
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
453
190
140
28
18
14
14
14
12
8
7
6
5
4
4
1
1
Host
Mouse
443
66
16
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Caspase 3 antibody is un-conjugated
24
21
17
10
9
9
4
3
2
2
2
2
2
2
2
1
1
Application
Fluorescence Microscopy (FM), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
368
193
160
88
62
58
36
25
23
21
13
10
7
3
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen Human CPP32 aa. 1-219
Clone 19-Caspase
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Apoptosis, a selective process of genetically programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptotic cells are characterized by loss of cell volume, plasma membrane blebbing, nuclear condensation, chromatin aggregation, and endonucleocytic degradation of DNA into nucleosomal fragments. Caspase-3 (CPP32, Yama, apopain) is a member of the family of cysteine proteases which includes interleukin-1beta converting enzyme (ICE) and C. elegans protein, Ced-3. An apoptotic signal such as granzyme B of cytotoxic T-cells (CTLs) or ICE-like proteases induces the intracellular cleavage of Caspase-3 from the inactive pro-form (32 kDa) to the active form which consists of the p20, p17, and p12 subunits. The active form of Caspase-3 cleaves several other apoptotic proteins including proteins such as DNA Fragmentation Factor (DFF). Apoptosis can be inhibited by coexpression of Bcl-2 as well as inhibitors of Caspase-3 or other members of the family of cysteine proteases. This antibody recognizes the human pro-form (inactive) of Caspase-3 at 32 kDa. In addition, it has been reported to recognize the active form of Caspase-3 at 20-21 kD in apoptotic cell lysates.
Synonyms: CPP32
Molecular Weight 32 kDa
Research Area Apoptosis/Necrosis, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis
Comment

Related Products: ABIN968537, ABIN967389, ABIN967299

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 1-219) (ABIN967902) Western blot analysis of Caspase-3 on a Jurkat cell lysate (Human T-cell leukemia, AT...
 image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 1-219) (ABIN967902) anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 1-219) antibody (Image 2)
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 1-219) (ABIN967902) anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 1-219) antibody (Image 3)
Product cited in: Takemoto, Nagai, Miyawaki et al.: "Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects." in: The Journal of cell biology, Vol. 160, Issue 2, pp. 235-43, 2003 (PubMed).

Li, Chen, Sinogeeva et al.: "Arsenic trioxide promotes histone H3 phosphoacetylation at the chromatin of CASPASE-10 in acute promyelocytic leukemia cells." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49504-10, 2002 (PubMed).

Suzuki, Nakabayashi, Takahashi: "Ubiquitin-protein ligase activity of X-linked inhibitor of apoptosis protein promotes proteasomal degradation of caspase-3 and enhances its anti-apoptotic effect in Fas-induced cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 15, pp. 8662-7, 2001 (PubMed).

Donoghue, Baden, Lauder et al.: "Immunohistochemical localization of caspase-3 correlates with clinical outcome in B-cell diffuse large-cell lymphoma." in: Cancer research, Vol. 59, Issue 20, pp. 5386-91, 1999 (PubMed).

Scaffidi, Fulda, Srinivasan et al.: "Two CD95 (APO-1/Fas) signaling pathways." in: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, 1998 (PubMed).

Background publications Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995 (PubMed).