Nitric Oxide Synthase 2, Inducible (NOS2) antibody

Details for Product No. ABIN967907
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Antigen
Synonyms NOS2A, INOS, iNOS, INO1, IPS, IPS 1, IPS-1, HEP-NOS, NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNos, NOS2, NOS2a
Reactivity
Mouse (Murine)
(156), (94), (62), (24), (24), (12)
Host
Rabbit
(169), (21)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(125), (77), (57), (46), (42), (39), (20), (19), (15), (12), (1), (1)
Pubmed 5 references available
Quantity 50 µg
Options
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Catalog No. ABIN967907
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Isotype Ig
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name iNOS/NOS Type II
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-gamma, TNF-alpha, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NF-kappaB. Data indicates that TGF-beta affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.
The immunogen used to generate this polyclonal antibody was mouse iNOS aa. 961-1144.
Molecular Weight 130 kDa
Comment

Related Products: ABIN968550, ABIN967390

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Nitric Oxide Synthase 2, Inducible (NOS2) antibody Western blot analysis of iNOS/NOS Type II on a cell lysate from mouse macrophages (RAW 264.7) stimulated with 10 ng/mL IFNgamma and 1 µg/mL LPS for 12 hours. 0.25 µg/mL (lane 1), 0.125 µg/mL (lane 2), 0.0625 µg/mL (lane 3) of the polyclonal rabbit anti-iNOS/NOS Type II antibody.
anti-Nitric Oxide Synthase 2, Inducible (NOS2) antibody (2) Immunofluorescence staining of 10 ng/mL IFNgamma and 1 µg/mL LPS stimulated mouse macrophages.
Product cited in: Xie, Cho, Calaycay et al.: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, 1992 (PubMed).

Coers, Timens, Kempinga et al.: "Specificity of antibodies to nitric oxide synthase isoforms in human, guinea pig, rat, and mouse tissues." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 46, Issue 12, pp. 1385-92, 1999 (PubMed).

Aiello, Noris, Piccinini et al.: "Thymic dendritic cells express inducible nitric oxide synthase and generate nitric oxide in response to self- and alloantigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 9, pp. 4649-58, 2000 (PubMed).

Mordue, Monroy, La Regina et al.: "Acute toxoplasmosis leads to lethal overproduction of Th1 cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4574-84, 2001 (PubMed).

Glynne, Darling, Picot et al.: "Epithelial inducible nitric-oxide synthase is an apical EBP50-binding protein that directs vectorial nitric oxide output." in: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 33132-8, 2002 (PubMed).

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