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E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 735-883)

Details for Product anti-CDH1 Antibody No. ABIN967949, Supplier: Log in to see
Antigen
  • AA960649
  • arc-1
  • Arc-1
  • Arc1
  • b-cadherin
  • cadherin-1
  • CD324
  • cd324
  • cdh1
  • cdh1-a
  • cdhc-A
  • cdhe
  • CDHE
  • cdhp
  • E-Cad
  • E-cad
  • E-cadherin
  • ecad
  • Ecad
  • ECAD
  • hjmd
  • L-CAM
  • l-cam
  • lcam
  • LCAM
  • pcad
  • si:dz180o5.2
  • Um
  • uvo
  • UVO
  • uvomorulin
  • xb-cad
  • XB-cadherin
  • XBcad
  • xcdh1
  • XTCAD-1
Epitope
AA 735-883
81
40
36
19
17
16
14
14
13
13
10
8
8
7
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Rat (Rattus)
621
161
95
25
20
12
6
5
4
3
3
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
376
262
26
18
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This E-cadherin antibody is un-conjugated
38
31
27
17
16
14
14
10
10
9
8
8
8
8
8
8
8
7
7
7
6
5
4
3
3
3
3
3
2
2
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
488
218
190
168
133
126
105
99
63
32
12
9
6
4
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen Human E-Cadherin aa. 735-883
Clone 34-E
Isotype IgG2b
Cross-Reactivity Dog (Canine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name E-Cadherin (CDH1 Antibody Abstract)
Background E-Cadherin is a 120kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. This antibody is routinely tested by western blot analysis.
Comment

Related Products: ABIN967389, ABIN968533

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 735-883) (ABIN967949) Western blot analysis of E-Cadherin on A431 cell lysate. Lane 1: 1:250, lane 2: 1:500...
Immunofluorescence (IF) image for anti-E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 735-883) (ABIN967949) Immunofluorescent staining of Human Fibroblast cells with anti-E-Cadherin antibody.
 image for anti-E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (AA 735-883) (ABIN967949) anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 735-883) antibody (Image 3)
Product cited in: Zundel, Swiersz, Giaccia: "Caveolin 1-mediated regulation of receptor tyrosine kinase-associated phosphatidylinositol 3-kinase activity by ceramide." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1507-14, 2000 (PubMed).

Huan, van Adelsberg: "Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins." in: The Journal of clinical investigation, Vol. 104, Issue 10, pp. 1459-68, 1999 (PubMed).

Chitaev, Troyanovsky: "Adhesive but not lateral E-cadherin complexes require calcium and catenins for their formation." in: The Journal of cell biology, Vol. 142, Issue 3, pp. 837-46, 1998 (PubMed).

Behrens, Vakaet, Friis, Winterhager, Van Roy, Mareel, Birchmeier et al.: "Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC ..." in: The Journal of cell biology, Vol. 120, Issue 3, pp. 757-66, 1993 (PubMed).

Takeichi: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." in: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, 1988 (PubMed).