Caveolin-1 antibody (AA 1-178)

Catalog No. ABIN967951

Product Details anti-Caveolin-1 Antibody

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Binding Specificity: AA 1-178

Reactivity: Human, Rat, Mouse, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: RSV-CEF Caveolin aa. 1-178

Clone: 2297-Caveolin 1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Caveolin-1 antibody (ABIN967951)

Woodman, Park, Cohen, Cheung, Chandra, Shirani, Tang, Jelicks, Kitsis, Christ, Factor, Tanowitz, Lisanti: "Caveolin-3 knock-out mice develop a progressive cardiomyopathy and show hyperactivation of the p42/44 MAPK cascade." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38988-97, (2002) (PubMed).

Ushio-Fukai, Hilenski, Santanam, Becker, Ma, Griendling, Alexander: "Cholesterol depletion inhibits epidermal growth factor receptor transactivation by angiotensin II in vascular smooth muscle cells: role of cholesterol-rich microdomains and focal adhesions in angiotensin II signaling." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48269-75, (2001) (PubMed).

Galbiati, Volonte, Brown, Weinstein, Ben-Zeev, Pestell, Lisanti: "Caveolin-1 expression inhibits Wnt/beta-catenin/Lef-1 signaling by recruiting beta-catenin to caveolae membrane domains." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23368-77, (2000) (PubMed).

Breton, Lisanti, Tyszkowski, McLaughlin, Brown: "Basolateral distribution of caveolin-1 in the kidney. Absence from H+-atpase-coated endocytic vesicles in intercalated cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 46, Issue 2, pp. 205-14, (1998) (PubMed).

Conrad, Smart, Ying, Anderson, Bloom: "Caveolin cycles between plasma membrane caveolae and the Golgi complex by microtubule-dependent and microtubule-independent steps." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 1, pp. 1421-33, (1996) (PubMed).

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: Caveolin 1 (CAV1 Products)

Synonyms: fc07c04 antibody, wu:fc07c04 antibody, Cav-1 antibody, bscl3 antibody, cav1 antibody, cav1a antibody, cgl3 antibody, vip21 antibody, cav1b antibody, caveolin-1 antibody, cav antibody, mstp085 antibody, Caveolin-1 antibody, CAV1 antibody, cav-1 antibody, Cav antibody, caveolin 1 antibody, BSCL3 antibody, CGL3 antibody, MSTP085 antibody, PPH3 antibody, VIP21 antibody, caveolin 1 antibody, caveolin 1 L homeolog antibody, caveolin 1 S homeolog antibody, caveolin 1, caveolae protein antibody, caveolin 1, caveolae protein, 22kDa antibody, Caveolin antibody, cav1 antibody, cav1.L antibody, cav1.S antibody, CAV1 antibody, Cav1 antibody, cav-1 antibody

Background: Identified as a tyrosine phosphorylated protein in Rous sarcoma virus-transformed chick embryo fibroblasts (CEF), caveolin is now known to be ubiquitously expressed. Caveolin (also known as VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. This transmembrane protein plays a structural role in these specializations. Caveolin is also present at the trans-Golgi network (TGN) and similar quantities are found in apically and basolaterally destined transport vesicles. Caveolin is part of a complex containing glycosylphosphatidylinositol (GPI)-linked molecules and cytoplasmic signaling proteins. Caveolin is a transmembrane adaptor molecule that can simultaneously recognize GPI-linked proteins and interact with downstream cytoplasmic signaling molecules, such as c-yes, Annexin II, and hetero-trimeric G proteins. Caveolin-1 can generate two forms, alpha and beta, due to alternate splicing of the mRNA. The alpha isoform has been reported to be observed at 24 kD and the beta isoform at 21 kD. Caveolin-1 forms large lipid-binding homo-oligomers which are believed to play a role in caveolae formation. It may also function as a scaffolding protein which concentrates andorganizes signaling molecules, a role supported by the fact that caveolin-1 interacts directly with inactive Ras and G-protein alpha subunits. This antibody is routinely tested by western blot analysis.

Molecular Weight: 21-24 kDa

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals