Phosphotyrosine antibody

Catalog No. ABIN967962

Product Details anti-Phosphotyrosine Antibody

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: PY69

Isotype: IgG2a

Application Details

Application Notes: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Aqueous buffered solution containing glycerol and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Phosphotyrosine antibody (ABIN967962)

Doronin, Shumay, Wang, Malbon: "Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15124-31, (2002) (PubMed).

Doronin, Wang Hy, Malbon: "Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor, creating a potent feedback inhibitor of its tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10698-703, (2002) (PubMed).

Moro, Dolce, Cabodi, Bergatto, Boeri Erba, Smeriglio, Turco, Retta, Giuffrida, Venturino, Godovac-Zimmermann, Conti, Schaefer, Beguinot, Tacchetti, Gaggini, Silengo, Tarone, Defilippi: "Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9405-14, (2002) (PubMed).

Yan, Deng, Friedman: "Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1555-63, (2001) (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, (1988) (PubMed).

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Products

Target Type: Amino Acid

Background: Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.