Nitric Oxide Synthase 2, Inducible (NOS2) (AA 961-1144) antibody

Details for Product No. ABIN967963
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Antigen
Synonyms NOS2A, INOS, iNOS, INO1, IPS, IPS 1, IPS-1, HEP-NOS, NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNos, NOS2, NOS2a
Epitope
AA 961-1144
(38), (14), (14), (8), (7), (6), (6), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(156), (94), (62), (24), (24), (12)
Host
Mouse
(169), (21)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
(125), (77), (57), (46), (42), (39), (20), (19), (15), (12), (1), (1)
Pubmed 5 references available
Quantity 50 µg
Options
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Catalog No. ABIN967963
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Immunogen Mouse iNOS
Clone 54
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name iNOS/NOS Type II
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-gamma, TNF-alpha, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFkappaB. Data indicates that TGF-beta affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. This antibody has been reported to cross-react with nNOS and eNOS.
Synonyms: NOS Type II
Molecular Weight 130 kDa
Comment

Related Products: ABIN968550, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 961-1144) antibody Western blot analysis of iNOS/NOS Type II on a lysate from mouse macrophages (RAW 264.7) stimulated with 10 ng/mL IFNgamma and 1 myg/mL LPS for 12 hours. Lane 1: 1:2000, lane 2: 1:4000, lane 3: 1:8000 dilution of the mouse anti-iNOS/NOS Type II antibody.
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 961-1144) antibody (2) Immunofluorescence staining of mouse macrophages stimulated with 10 ng/mL IFNgamma and 1 µg/mL LPS.
Product cited in: Xie, Cho, Calaycay et al.: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, 1992 (PubMed).

Vodovotz, Bogdan, Paik et al.: "Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 605-13, 1993 (PubMed).

Zhao, Dugas, Mathiot et al.: "B-cell chronic lymphocytic leukemia cells express a functional inducible nitric oxide synthase displaying anti-apoptotic activity." in: Blood, Vol. 92, Issue 3, pp. 1031-43, 1998 (PubMed).

Resta, ODonaughy, Earley et al.: "Unaltered vasoconstrictor responsiveness after iNOS inhibition in lungs from chronically hypoxic rats." in: The American journal of physiology, Vol. 276, Issue 1 Pt 1, pp. L122-30, 1999 (PubMed).

Zadeh, Kolb, Geromin et al.: "Regulation of ICAM-1/CD54 expression on human endothelial cells by hydrogen peroxide involves inducible NO synthase." in: Journal of leukocyte biology, Vol. 67, Issue 3, pp. 327-34, 2000 (PubMed).

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