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Nitric Oxide Synthase 2, Inducible (NOS2) (AA 961-1144) antibody

Details for Product No. ABIN967963, Supplier: Log in to see
Antigen
  • NOS2A
  • INOS
  • iNOS
  • INO1
  • IPS
  • IPS 1
  • IPS-1
  • HEP-NOS
  • NOS
  • NOS-II
  • Nos-2
  • Nos2a
  • i-NOS
  • iNos
  • NOS2
  • NOS2a
Alternatives
anti-Mouse (Murine) Nitric Oxide Synthase 2, Inducible antibody for Immunocytochemistry
Epitope
AA 961-1144
46
28
15
13
13
13
11
10
9
7
6
6
5
5
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
216
130
67
13
3
Host
Mouse
238
47
6
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
12
11
5
5
5
4
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
208
132
76
67
51
37
32
22
19
18
11
6
3
2
1
1
1
1
1
Supplier
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Immunogen Mouse iNOS aa. 961-1144
Clone 54-iNOS
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name iNOS/NOS Type II (NOS2 Antibody Abstract)
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-gamma, TNF-alpha, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFkappaB. Data indicates that TGF-beta affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. This antibody has been reported to cross-react with nNOS and eNOS.
Synonyms: NOS Type II
Molecular Weight 130 kDa
Pathways Retinoic Acid Receptor Signaling Pathway
Comment

Related Products: ABIN968550, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 961-1144) antibody (ABIN967963) Western blot analysis of iNOS/NOS Type II on a lysate from mouse macrophages (RAW 264...
Immunofluorescence (IF) image for anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 961-1144) antibody (ABIN967963) Immunofluorescence staining of mouse macrophages stimulated with 10 ng/mL IFNgamma an...
Product cited in: Zadeh, Kolb, Geromin et al.: "Regulation of ICAM-1/CD54 expression on human endothelial cells by hydrogen peroxide involves inducible NO synthase." in: Journal of leukocyte biology, Vol. 67, Issue 3, pp. 327-34, 2000 (PubMed).

Resta, ODonaughy, Earley et al.: "Unaltered vasoconstrictor responsiveness after iNOS inhibition in lungs from chronically hypoxic rats." in: The American journal of physiology, Vol. 276, Issue 1 Pt 1, pp. L122-30, 1999 (PubMed).

Zhao, Dugas, Mathiot et al.: "B-cell chronic lymphocytic leukemia cells express a functional inducible nitric oxide synthase displaying anti-apoptotic activity." in: Blood, Vol. 92, Issue 3, pp. 1031-43, 1998 (PubMed).

Vodovotz, Bogdan, Paik et al.: "Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 605-13, 1993 (PubMed).

Xie, Cho, Calaycay et al.: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, 1992 (PubMed).