Synaptotagmin (AA 72-223) antibody

Details for Product No. ABIN967966
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Antigen
Synonyms Syti, GB20036, DKFZP459P193, SYT1, SytI
Epitope
AA 72-223
(15), (13), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(53), (49), (45), (6), (5), (4), (2), (2), (2)
Host
Mouse
(55), (3)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(49), (40), (23), (9), (3), (3), (2), (2), (2)
Pubmed 6 references available
Catalog no. ABIN967966
Quantity 150 µg
Price
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Immunogen Rat Synaptotagmin
Clone BC17
Isotype IgG1
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Background Synaptotagmin (p65) is an abundant synaptic vesicle protein that contains a single transmembrane region and two copies of an internal repeat that is homologous to the regulatory region of Protein Kinase C. It appears that synaptotagmin has a regulatory role in the synaptic vesicle pathway, particularly in vesicle docking and/or fusion with the plasmalemma. A model has been proposed to explain docking, activation, and fusion of synaptic vesicles with donor membranes. This model suggests that VAMP/synaptobrevin and synaptotagmin (vSNARE) on the synaptic vesicle, and SNAP-25 and syntaxin (tSNAREs) on the plasma membrane, interact to form a 7S complex. Two additional soluble proteins, alphaSNAP and NSF, are later added to the 7S complex, accompanied by the loss of synaptotagmin. The resulting 20S complex contains syntaxin, SNAP-25, VAMP, alphaSNAP, and NSF. Genetic studies in several species demonstrate that mutation or deletion of synaptotagmin results in a large decrease in Ca2+ triggered transmitter release. Mammalian synapses that lack synaptotagmin show a selective decrease in a fast component of release, suggesting that synaptotagmin is the Ca2+ sensor triggering exocytosis.
Molecular Weight 65 kDa
Research Area Cancer
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Synaptotagmin (AA 72-223) antibody Western blot analysis of Synaptotagmin on rat brain lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Synaptotagmin.
anti-Synaptotagmin (AA 72-223) antibody (2) anti-Synaptotagmin (AA 72-223) antibody (Image 2)
Product cited in: Perin, Johnston, Ozcelik et al.: "Structural and functional conservation of synaptotagmin (p65) in Drosophila and humans." in: The Journal of biological chemistry, Vol. 266, Issue 1, pp. 615-22, 1991 (PubMed).

Scheller: "Membrane trafficking in the presynaptic nerve terminal." in: Neuron, Vol. 14, Issue 5, pp. 893-7, 1995 (PubMed).

Duncan, Don-Wauchope, Tapechum et al.: "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells." in: The Biochemical journal, Vol. 342 Pt 3, pp. 497-501, 1999 (PubMed).

Ramalho-Santos, Moreno, Sutovsky et al.: "SNAREs in mammalian sperm: possible implications for fertilization." in: Developmental biology, Vol. 223, Issue 1, pp. 54-69, 2000 (PubMed).

Liu, Fallon, Lashuel et al.: "The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility." in: Cell, Vol. 111, Issue 2, pp. 209-18, 2002 (PubMed).

General Connolly, Chait, Duncan et al.: "CT-guided percutaneous needle biopsy of small lung nodules in children." in: Pediatric radiology, Vol. 29, Issue 5, pp. 342-6, 1999 (PubMed).

Hosts (55), (3)
Reactivities (53), (49), (45), (6), (5), (4), (2), (2), (2)
Applications (49), (40), (23), (9), (3), (3), (2), (2), (2)
Epitopes (15), (13), (2), (2), (2), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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