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Syntaxin 4 antibody (AA 1-280)

STX4 Reactivity: Human WB, IP, IF, IHC Host: Mouse Monoclonal 49-Syntaxin 4 unconjugated
Catalog No. ABIN967968
  • Target See all Syntaxin 4 (STX4) Antibodies
    Syntaxin 4 (STX4)
    Binding Specificity
    • 11
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-280
    Reactivity
    • 44
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 41
    • 5
    Mouse
    Clonality
    • 41
    • 5
    Monoclonal
    Conjugate
    • 27
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    This Syntaxin 4 antibody is un-conjugated
    Application
    • 33
    • 20
    • 8
    • 7
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to us.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Syntaxin 4 aa. 1-280
    Clone
    49-Syntaxin 4
    Isotype
    IgG1
    Top Product
    Discover our top product STX4 Primary Antibody
  • Comment

    Related Products: ABIN968536, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Faigle, Colucci-Guyon, Louvard, Amigorena, Galli: "Vimentin filaments in fibroblasts are a reservoir for SNAP23, a component of the membrane fusion machinery." in: Molecular biology of the cell, Vol. 11, Issue 10, pp. 3485-94, (2000) (PubMed).

    Ramalho-Santos, Moreno, Sutovsky, Chan, Hewitson, Wessel, Simerly, Schatten: "SNAREs in mammalian sperm: possible implications for fertilization." in: Developmental biology, Vol. 223, Issue 1, pp. 54-69, (2000) (PubMed).

    Kee, Lin, Hsu, Scheller: "Distinct domains of syntaxin are required for synaptic vesicle fusion complex formation and dissociation." in: Neuron, Vol. 14, Issue 5, pp. 991-8, (1995) (PubMed).

    Bennett, Calakos, Scheller: "Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones." in: Science (New York, N.Y.), Vol. 257, Issue 5067, pp. 255-9, (1992) (PubMed).

  • Target
    Syntaxin 4 (STX4)
    Alternative Name
    Syntaxin 4 (STX4 Products)
    Synonyms
    CG2715 antibody, Dm Syx4 antibody, DmSyx4 antibody, Dmel\\CG2715 antibody, EG:95B7.1 antibody, EG:95B7.11 antibody, Syx antibody, Syx-4 antibody, syx4 antibody, STX4 antibody, p35-2 antibody, stx4a antibody, syntaxin4 antibody, STX4A antibody, Stx4a antibody, Stx4 antibody, Syn-4 antibody, Syn4 antibody, Syntaxin 4 antibody, syntaxin 4 antibody, syntaxin 4 L homeolog antibody, syntaxin 4A (placental) antibody, Syx4 antibody, STX4 antibody, stx4 antibody, stx4.L antibody, Stx4 antibody, Stx4a antibody
    Background
    Signal transmission between neurons is regulated by the release of neurotransmitters at the synapse. This process is controlled by a complex pathway of membrane trafficking in the presynaptic nerve terminal, which leads to membrane fusion and subsequent neurotransmitter secretion. Syntaxin is involved in three important protein complexes that modulate this process: syntaxin and n-sec1, syntaxin, VAMP and SNAP-25, and syntaxin, VAMP, SNAP-25, alphaSNAP, and NSF (20S complex). A model has been proposed to explain docking, activation, and fusion of synaptic vesicles with donor membranes. This model suggests that VAMP/synaptobrevin and synaptotagmin (vSNARE) on the synaptic vesicle, and SNAP-25 and syntaxin (tSNAREs) on the plasma membrane, interact to form a 7S complex. It appears that syntaxin associates with -sec1 prior to and/or during the formation of the 7S complex. Two additional soluble proteins, alphaSNAP and NSF, associate with this complex as synaptotagmin releases from the complex. The resulting 20S complex contains syntaxin, SNAP-25, VAMP, alphaSNAP, and NSF. A large region including the N-terminus is involved in binding n-sec1 while both VAMP and SNAP-25 bind within residues 199-288 of syntaxin.
    Synonyms: STX4
    Molecular Weight
    32 kDa
    Pathways
    Synaptic Vesicle Exocytosis
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