Casein Kinase 2, beta (CSNK2B) (AA 21-215) antibody

Antigen: Casein Kinase 2, beta (CSNK2B)

Synonyms: G5A, CK2B, CK2N, CSK2B, MGC138222, MGC138224, Ck2b, BmCK2b, csnk2b, wu:fa16g01

Binding Site: AA 21-215

Clonality: Monoclonal (51)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967973

Additional Information

Alternative name: Casein Kinase IIbeta

Immunogen: Human Casein Kinase IIbeta

Cross-Reactivity: Rat (Rattus), Dog (Canine), Mouse (Murine), Insect, Rabbit

Format: Liquid

Isotype: IgG1

Clone: 51

Description: Casein Kinase II (CKII) is a serine/threonine protein kinase that is widely distributed in eukaryotic cells. The CKII holoenzyme exists as the oligomeric structures: alpha2beta2 or alphaalpha'beta2 The alpha and alpha' subunits contain catalytic sites, while the beta subunit contains a site for autophosphorylation. A 1:1 stoichiometry of the alpha and beta subunits is required for optimal CKII activity and it is thought that autophosphorylation of the beta subunit plays a role in regulating the holoenzyme. CKII has been shown to have multiple roles within the cell. In G1, CKII phosphorylates p34[cdc]2, and maintains it in a monomeric state unbound from Cyclin A. CKII is also required for transition of G0/G1, early G1, G1/S and G2/M phases of the cell cycle. In addition, CKII is a major c-Jun kinase. CKII phosphorylation of c-Jun inhibits its DNA binding ability resulting in attenuated activity of Jun/AP-1. CKII is also found associated with the nuclear matrix, indicating a possible role in nuclear matrix protein phosphorylation.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 25 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of Human Casein Kinase IIbeta on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-human Casein Kinase IIbeta.

Immunofluorescent staining of rat neurons.

anti-Casein Kinase 2, beta (CSNK2B) (AA 21-215) antibody (Image 3)

Publications

Product: Hanna, Rethinaswamy, Glover: "Casein kinase II is required for cell cycle progression during G1 and G2/M in Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 270, Issue 43, pp. 25905-14, 1995 (PubMed).

Pepperkok, Lorenz, Ansorge et al.: "Casein kinase II is required for transition of G0/G1, early G1, and G1/S phases of the cell cycle." in: The Journal of biological chemistry, Vol. 269, Issue 9, pp. 6986-91, 1994 (PubMed).

Tawfic, Ahmed: "Association of casein kinase 2 with nuclear matrix. Possible role in nuclear matrix protein phosphorylation." in: The Journal of biological chemistry, Vol. 269, Issue 10, pp. 7489-93, 1994 (PubMed).

Keller, Lu: "p53 serine 392 phosphorylation increases after UV through induction of the assembly of the CK2.hSPT16.SSRP1 complex." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 50206-13, 2002 (PubMed).