MAP2 antibody (AA 19-219)

Catalog No. ABIN967986

Product Details anti-MAP2 Antibody

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Binding Specificity: AA 19-219

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human MAP2B aa. 19-219

Clone: 18-MAP2B

Isotype: IgG1 kappa

Application Details

Application Notes: Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-MAP2 antibody (ABIN967986)

Kanaani, el-Husseini, Aguilera-Moreno, Diacovo, Bredt, Baekkeskov: "A combination of three distinct trafficking signals mediates axonal targeting and presynaptic clustering of GAD65." in: The Journal of cell biology, Vol. 158, Issue 7, pp. 1229-38, (2002) (PubMed).

Kindler, Schulz, Goedert, Garner: "Molecular structure of microtubule-associated protein 2b and 2c from rat brain." in: The Journal of biological chemistry, Vol. 265, Issue 32, pp. 19679-84, (1991) (PubMed).

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP2B (MAP2 Products)

Synonyms: MAP2A antibody, MAP2B antibody, MAP2C antibody, MAP2R antibody, Mtap2 antibody, MAP-2 antibody, Mtap-2 antibody, xmap2 antibody, MGC53131 antibody, a730034c02 antibody, zgc:103474 antibody, zgc:113974 antibody, microtubule associated protein 2 antibody, microtubule-associated protein 2 antibody, microtubule associated protein 2 S homeolog antibody, MAP2 antibody, Map2 antibody, map2.S antibody, map2 antibody

Background: Microtubule-associated proteins (MAPs) play a crucial role in the development and structure of nerve cells. These proteins are important for the assembly and stability of microtubules during neurite outgrowth and for the morphology of neuronal processes, such as dendrites, MAP2, specifically localized in dendrites, has four known isoforms produced by alternative splicing of the transcript. These isoforms, MAPs A, B, C, and D, are expressed at various stages of neuronal development. MAP2B is a 280-kDa protein expressed throughout brain development. It is composed of several highly conserved domains that are flanked by domains with extensive sequence divergence. An N-terminal conserved domain overlaps with a binding domain for the regulatory subunit of the cAMP-dependent kinase II, while a C-terminal conserved domain overlaps with a microtubule-binding domain. Secondary structure prediction suggests that the portion of MAP2B extending from the microtubule surface is composed of a number of alpha-helices separated by small turns which may account for the extended, yet flexible, structure of MAP2B.

Molecular Weight: 280 kDa