General Transcription Factor IIB (GTF2B) (AA 2-155) antibody

Antigen: General Transcription Factor IIB (GTF2B)

Synonyms: GTL3, EVORF, fSAP23, MGC6859, zgc:66258, wu:fc44c11, MGC73380, gtf2b-A, XLTFIIB, GTF2B, MGC137096, DKFZp468I1319, TF2B, TFIIB

Binding Site: AA 2-155

Clonality: Monoclonal (24)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN967988

Additional Information

Alternative name: TFIIB

Immunogen: Human TFIIB

Cross-Reactivity: Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG2a

Clone: 24

Description: TFIIB is a ubiquitous factor required for transcription initiation by RNA polymerase II. Studies suggest that TFIIB serves as a bridge between the TATA-binding factor (TFIID) and RNA polymerase II during pre-initiation complex assembly and that TFIIB can be a target of acidic activators. An essential component of the machinery that transcribes protein-coding genes, the three-dimensional structure of the human TFIIB appears to consist of two direct repeats that adopt similar alpha-helical folds, conferring pseudo-twofold symmetry. An extensive, central basic surface, including an amphipathic alpha helix, is critical to the function of TFIIB as a bridge between the TBP-promoter complex and transcription factors. TFIIB has a calculated molecular weight of 33 kD and has been reported to be observed to migrate between 33-40 kD. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 38 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of TFIIB on a A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti-TFIIB antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).

Publications

Product: Malik, Hisatake, Sumimoto et al.: "Sequence of general transcription factor TFIIB and relationships to other initiation factors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 21, pp. 9553-7, 1991 (PubMed).

Bagby, Kim, Maldonado et al.: "Solution structure of the C-terminal core domain of human TFIIB: similarity to cyclin A and interaction with TATA-binding protein." in: Cell, Vol. 82, Issue 5, pp. 857-67, 1995 (PubMed).

Pellizzoni, Charroux, Rappsilber et al.: "A functional interaction between the survival motor neuron complex and RNA polymerase II." in: The Journal of cell biology, Vol. 152, Issue 1, pp. 75-85, 2001 (PubMed).

Lamberti, Lin, Yamamoto et al.: "Regulation of beta-catenin function by the IkappaB kinases." in: The Journal of biological chemistry, Vol. 276, Issue 45, pp. 42276-86, 2001 (PubMed).