Dynactin 1 antibody (AA 3-202)

Catalog No. ABIN967995

Product Details anti-Dynactin 1 Antibody

Target: Dynactin 1 (DCTN1)

Binding Specificity: AA 3-202

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dynactin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Cross-Reactivity: Human, Chicken, Dog (Canine), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat p150 [Glued] aa. 3-202

Clone: 1-p150Glued

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Dynactin 1 antibody (ABIN967995)

Kurzchalia: "Anthrax toxin rafts into cells." in: The Journal of cell biology, Vol. 160, Issue 3, pp. 295-6, (2003) (PubMed).

Askham, Vaughan, Goodson, Morrison: "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3627-45, (2002) (PubMed).

Aumais, Tunstead, McNeil, Schaar, McConnell, Lin, Clark, Yu-Lee: "NudC associates with Lis1 and the dynein motor at the leading pole of neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 24, pp. RC187, (2001) (PubMed).

Tai, Chuang, Sung: "Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport." in: The Journal of cell biology, Vol. 153, Issue 7, pp. 1499-509, (2001) (PubMed).

King, Schroer: "Dynactin increases the processivity of the cytoplasmic dynein motor." in: Nature cell biology, Vol. 2, Issue 1, pp. 20-4, (2000) (PubMed).

Target Details for Dynactin 1

Target: Dynactin 1 (DCTN1)

Alternative Name: p150 Glued (DCTN1 Products)

Synonyms: DAP-150 antibody, DP-150 antibody, P135 antibody, Chip150 antibody, DCTN1 antibody, DKFZp469C1813 antibody, AL022633 antibody, Glued antibody, p150 antibody, p150 antibody, CG9206 antibody, DYNA_DROME antibody, Dmel\\CG9206 antibody, FCP-D antibody, Glu antibody, P150 antibody, P150[Glued] antibody, p150/Glued antibody, p150/glued antibody, p150[Glued] antibody, p150[glued] antibody, t1 antibody, Dynactin-1 antibody, glued antibody, dynactin subunit 1 antibody, dynactin 1 antibody, Dynactin 1, p150 subunit antibody, dynactin subunit 1 L homeolog antibody, DCTN1 antibody, Dctn1 antibody, DCTN1-p150 antibody, dctn1.L antibody

Background: P150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein, the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule bindings assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association. This antibody is routinely tested by western blot analysis.

Molecular Weight: 150 kDa

Pathways: M Phase, ER-Nucleus Signaling