Tumor Protein, Translationally-Controlled 1 (TPT1) (AA 91-107) antibody

Antigen: Tumor Protein, Translationally-Controlled 1 (TPT1)

Synonyms: HRF, p02, TCTP, FLJ27337, Trt, TCT1, tctp, fa28a02, id:ibd2078, wu:fa28a02, MGC105360, MGC128232, tpt1, MGC53129, PCHK23, TPT1, hrf, MGC79720, Tpt1

Binding Site: AA 91-107

Clonality: Monoclonal (20)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN967997

Additional Information

Alternative name: HRF

Immunogen: Human HRF

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 20

Description: One of the most powerful effector mechanisms of the immune system is the reaction initiated by IgE-dependent tissue stimulation of mast cells and basophils. When antigen binds surface IgE on these cells, there is a rapid release of histamine and other mediators that collectively initiate immediate hypersensitivity. This effect is part of a larger response called the late phase reaction (LPR). An IgE-dependent Histamine Releasing Factor (HRF) is present in biological fluids of allergic patients. It causes the release of histamine in IgE-producing cell preparations, where non IgE-producing cells of the same origin do not release histamine. HRF has been reported to be observed as a 21 kDa or 23 kDa protein produced in mouse and human cell lines, respectively. Its sequence does not predict a secretory form, but HRF is found in biological fluids. Neither the exact function of this protein nor the method of its secretion is known.
Synonyms: Histamine Releasing Factor

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 23 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of HRF on a A431 cell lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-HRF antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).

anti-Tumor Protein, Translationally-Controlled 1 (TPT1) (AA 91-107) antibody (Image 3)

Publications

Product: MacDonald, Rafnar, Langdon et al.: "Molecular identification of an IgE-dependent histamine-releasing factor." in: Science (New York, N.Y.), Vol. 269, Issue 5224, pp. 688-90, 1995 (PubMed).