Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2) (AA 254-497) antibody
|Synonyms||RCH2, SRP1, IPOA5, NPI-1, QIP2, RCH1, IPOA1, SRP1alpha, Gm1055, AW146299, KPNA1, DKFZp459M124, ima2|
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
|4 references available|
|Quantity||150 µg (250 µg/ml) (Variants)|
|Price||Product not available in this region.|
|Alternative name||Karyopherin alpha 2|
|Cross-Reactivity||Mouse (Murine), Rat (Rattus), Dog (Canine), Fruit Fly (Drosophila melanogaster)|
The two step process of importing proteins into the nucleus involves the binding and interaction of several cytosolic and nuclear pore proteins. Proteins to be translocated into the nucleus contain a nuclear localization sequence (NLS) which is recognized and bound by carrier proteins in the cytosol. Heterodimers belonging to a highly conserved family of proteins called karyopherins are required for successful nuclear localization of cytosolic proteins. The alpha-subunits appear to function in the binding of NLS (both simple and bitartite NLS motifs), but both alpha- and beta-subunits are required for successful docking to the nuclear envelope. ATP is required for complete translocation of proteins into the nucleus. Karyopherin alpha2 was first identified as Rch-1, an NLS receptor which interacts with the RAG-1 recombination-activating protein in developing B and T cells. Rch-1 has been reported to be 44% identical to karyopherin alpha1 (hSRP-1 /NPI-1). This antibody is routinely tested by western blot analysis.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||58 kDa|
Related Products: ABIN968535, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Moroianu, Hijikata, Blobel et al.: "Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 14, pp. 6532-6, 1995 (PubMed).
Weis, Mattaj, Lamond: "Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences." in: Science (New York, N.Y.), Vol. 268, Issue 5213, pp. 1049-53, 1995 (PubMed).
Cuomo, Kirch, Gyuris et al.: "Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 13, pp. 6156-60, 1994 (PubMed).
Grozinger, Schreiber: "Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7835-40, 2000 (PubMed).
|Hosts||Rabbit (18), Goat (4), Rat (3), Sheep (1)|
|Reactivities||Human (24), Mouse (Murine) (6), Rat (Rattus) (6), Dog (Canine) (3), Cow (Bovine) (2), Cat (Feline) (1), Chicken (1), Pig (Porcine) (1)|
|Applications||Western Blotting (WB) (25), ELISA (15), Immunohistochemistry (IHC) (5), Immunofluorescence (IF) (3), Immunoprecipitation (IP) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Dot Blot (DB) (1), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1)|
|Epitopes||N-Term (4), Internal Region (2), AA 1-132 (1), AA 116-515 (1), C-Term (1), Center (1)|