Zyxin (ZYX) (AA 277-447) antibody

Antigen: Zyxin (ZYX)

Synonyms: ESP-2, HED-2, R75157, 9530098H06Rik, BcDNA:LD06023, DLim-1, DmLIM-1, Lim102EF, ZYX102, Zyxin, lim, DmelCG32018, CG32018, ZYX, MGC142604, zyx, MGC145270, LOC100049724

Binding Site: AA 277-447

Clonality: Monoclonal (21)

Host: Mouse

Reactivity: Chicken

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968016

Additional Information

Alternative name: Zyxin

Immunogen: Chicken Zyxin

No Cross-Reactivity: Human, Dog (Canine), Rat (Rattus), Mouse (Murine), Frog

Format: Liquid

Isotype: IgG1

Clone: 21

Description: Zyxin is an 82kDa zinc-binding metalloprotein found in focal adhesion plaques. It contains a proline-rich N-terminal domain and a C-terminal domain containing three LIM domains. LIM domains are characterized as zinc-binding motifs involved in gene expression protein-protein interactions. Zyxin interacts with several focal adhesion proteins including alpha-actinin, Vav, and another LIM domain-containing protein, cCRP. Zyxin associates selectively with an SH3 domain of p95 Vav through its proline-rich N-terminal domain. The focal adhesion protein VASP has also been shown to interact with the 83kDa human homologue of zyxin, p83. Other LIM-containing proteins function as transcription factors or as growth and differentiation factors in embryogenesis. Zyxin and its homologues appear to be involved in the transduction of extracellular signals originating at focal adhesion plaques.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 83 kDa

Comments:

Related Products: ABIN968554, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Cytoskeleton, Microfilaments

Restrictions: For Research Use only

Images

Western blot analysis of Zyxin on SL-29 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-Zyxin.

Immunofluorescent staining of chick fibroblasts.

anti-Zyxin (ZYX) (AA 277-447) antibody (Image 3)

Publications

Product: Sadler, Crawford, Michelsen et al.: "Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton." in: The Journal of cell biology, Vol. 119, Issue 6, pp. 1573-87, 1993 (PubMed).

Reinhard, Jouvenal, Tripier et al.: "Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7956-60, 1995 (PubMed).

Hobert, Schilling, Beckerle et al.: "SH3 domain-dependent interaction of the proto-oncogene product Vav with the focal contact protein zyxin." in: Oncogene, Vol. 12, Issue 7, pp. 1577-81, 1996 (PubMed).

Frischknecht, Cudmore, Moreau et al.: "Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella." in: Current biology : CB, Vol. 9, Issue 2, pp. 89-92, 1999 (PubMed).

Drees, Friederich, Fradelizi et al.: "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22503-11, 2000 (PubMed).