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Zyxin (ZYX) (AA 277-447) antibody

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Synonyms BcDNA:LD06023, CG32018, DLim-1, DmLIM-1, Dmel\\CG32018, Lim102EF, ZYX102, Zyx102, Zyx102EF, lim, zyx102, zyxin, zgc:171680, ZYX, zyx, MGC145270, ESP-2, HED-2, 9530098H06Rik, R75157
AA 277-447
(8), (8), (8), (7), (7), (7), (5), (3), (2), (1), (1), (1), (1)
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(85), (17), (15), (4), (4), (2), (1), (1), (1), (1), (1)
(53), (18), (14)
Clonality (Clone)
Monoclonal ()
(4), (4), (2), (1), (1), (1), (1)
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(74), (46), (15), (12), (10), (8), (4), (3), (3), (2), (1), (1)
Pubmed 5 references available
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Quantity 50 μg
Shipping to United States ( )
Immunogen Chicken Zyxin aa. 277-447
Clone 21-Zyxin
Isotype IgG1
No Cross-Reactivity Human, Dog (Canine), Rat (Rattus), Mouse (Murine), Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Zyxin (ZYX Antibody Abstract)
Background Zyxin is an 82kDa zinc-binding metalloprotein found in focal adhesion plaques. It contains a proline-rich N-terminal domain and a C-terminal domain containing three LIM domains. LIM domains are characterized as zinc-binding motifs involved in gene expression protein-protein interactions. Zyxin interacts with several focal adhesion proteins including alpha-actinin, Vav, and another LIM domain-containing protein, cCRP. Zyxin associates selectively with an SH3 domain of p95 Vav through its proline-rich N-terminal domain. The focal adhesion protein VASP has also been shown to interact with the 83kDa human homologue of zyxin, p83. Other LIM-containing proteins function as transcription factors or as growth and differentiation factors in embryogenesis. Zyxin and its homologues appear to be involved in the transduction of extracellular signals originating at focal adhesion plaques.
Molecular Weight 83 kDa
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Cytoskeleton, Microfilaments

Related Products: ABIN968554, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Zyxin (ZYX) (AA 277-447) antibody (ABIN968016) Western blot analysis of Zyxin on SL-29 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, ...
Immunofluorescence (IF) image for anti-Zyxin (ZYX) (AA 277-447) antibody (ABIN968016) Immunofluorescent staining of chick fibroblasts.
 image for anti-Zyxin (ZYX) (AA 277-447) antibody (ABIN968016) anti-Zyxin (ZYX) (AA 277-447) antibody (Image 3)
Western Blotting (WB) image for anti-Zyxin (ZYX) (AA 277-447) antibody (ABIN968016) anti-Zyxin (ZYX) (AA 277-447) antibody (Image 4)
Product cited in: Drees, Friederich, Fradelizi et al.: "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22503-11, 2000 (PubMed).

Frischknecht, Cudmore, Moreau et al.: "Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella." in: Current biology : CB, Vol. 9, Issue 2, pp. 89-92, 1999 (PubMed).

Hobert, Schilling, Beckerle et al.: "SH3 domain-dependent interaction of the proto-oncogene product Vav with the focal contact protein zyxin." in: Oncogene, Vol. 12, Issue 7, pp. 1577-81, 1996 (PubMed).

Reinhard, Jouvenal, Tripier et al.: "Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7956-60, 1995 (PubMed).

Sadler, Crawford, Michelsen et al.: "Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton." in: The Journal of cell biology, Vol. 119, Issue 6, pp. 1573-87, 1993 (PubMed).

Catalog No. ABIN968016
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