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Ankyrin Repeat and Sterile alpha Motif Domain Containing 1B (ANKS1B) (AA 107-268) antibody

Details for Product No. ABIN968021, Supplier: Log in to see
Antigen
  • AIDA
  • AIDA-1
  • AIDA-1b
  • ANKS2
  • C030032C09Rik
  • cajalin-2
  • COX7AR
  • COX7RP
  • E530015N03Rik
  • EB-1
  • EB1
  • ENSMUSG00000078431
  • Gm1555
  • Gm10937
  • RGD1565556
  • si:ch211-146a9.3
  • si:dkey-11k24.3
  • SIG81
Epitope
AA 107-268
15
14
10
5
3
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
44
20
18
1
1
Host
Mouse
42
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
34
18
17
11
7
3
3
Supplier
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Immunogen Human EB1 aa. 107-268
Clone 5-EB1
Isotype IgG1
Cross-Reactivity Chicken, Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name EB1 (ANKS1B Antibody Abstract)
Background Familial adenomatous polyposis is an autosomal inherited disease characterized by mutations in the APC (adenomatous polyposis coli) gene. Individuals with this disease are predisposed to colorectal polyps and cancer. It has been proposed that disruption of the APC gene plays a role in the disease. APC is a large coiled-coil protein that interacts, via its carboxy terminus, with catenins and with the protein, EB1. This 30 kDa protein was discovered as a result of its strong binding to the C-terminal domain of APC. Like the APC-binding proteins beta-Catenin and pp120Cas, EB1 may play an essential role in modulating APC activity and tumorigenesis. This antibody is routinely tested by western blot analysis.
Molecular Weight 30 kDa
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Ankyrin Repeat and Sterile alpha Motif Domain Containing 1B (ANKS1B) (AA 107-268) antibody (ABIN968021) Western blot analysis of EB1 on a human endothelial cell lysate. Lane 1: 1:500, lane ...
Immunofluorescence (IF) image for anti-Ankyrin Repeat and Sterile alpha Motif Domain Containing 1B (ANKS1B) (AA 107-268) antibody (ABIN968021) Immunofluorescence staining of human intestinal smooth muscle cells (HISM).
Product cited in: Etienne-Manneville, Hall: "Cdc42 regulates GSK-3beta and adenomatous polyposis coli to control cell polarity." in: Nature, Vol. 421, Issue 6924, pp. 753-6, 2003

Askham, Vaughan, Goodson, Morrison: "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3627-45, 2002

Komarova, Akhmanova, Kojima, Galjart, Borisy: "Cytoplasmic linker proteins promote microtubule rescue in vivo." in: The Journal of cell biology, Vol. 159, Issue 4, pp. 589-99, 2002

Mamane, Grandvaux, Hernandez, Sharma, Innocente, Lee, Azimi, Lin, Hiscott: "Repression of IRF-4 target genes in human T cell leukemia virus-1 infection." in: Oncogene, Vol. 21, Issue 44, pp. 6751-65, 2002

Su, Huang, Fair, Powell, Heston: "Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio of expression as a potential measurement of progression." in: Cancer research, Vol. 55, Issue 7, pp. 1441-3, 1995