Ankyrin Repeat and Sterile alpha Motif Domain Containing 1B (ANKS1B) (AA 107-268) antibody

Antigen: Ankyrin Repeat and Sterile alpha Motif Domain Containing 1B (ANKS1B)

Binding Site: AA 107-268

Clonality: Monoclonal (5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe))

Catalog no.: ABIN968022

Additional Information

Alternative name: EB1

Immunogen: Human EB1

Cross-Reactivity: Chicken, Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 5

Description: Familial adenomatous polyposis is an autosomal inherited disease characterized by mutations in the APC (adenomatous polyposis coli) gene. Individuals with this disease are predisposed to colorectal polyps and cancer. It has been proposed that disruption of the APC gene plays a role in the disease. APC is a large coiled-coil protein that interacts, via its carboxy terminus, with catenins and with the protein, EB1. This 30 kDa protein was discovered as a result of its strong binding to the C-terminal domain of APC. Like the APC-binding proteins beta-Catenin and pp120Cas, EB1 may play an essential role in modulating APC activity and tumorigenesis. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 30 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of EB1 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- EB1 antibody.

Immunofluorescence staining of human intestinal smooth muscle cells (HISM).

Publications

Product: Su, Huang, Fair et al.: "Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio of expression as a potential measurement of progression." in: Cancer research, Vol. 55, Issue 7, pp. 1441-3, 1995 (PubMed).

Mamane, Grandvaux, Hernandez et al.: "Repression of IRF-4 target genes in human T cell leukemia virus-1 infection." in: Oncogene, Vol. 21, Issue 44, pp. 6751-65, 2002 (PubMed).

Askham, Vaughan, Goodson et al.: "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3627-45, 2002 (PubMed).

Komarova, Akhmanova, Kojima et al.: "Cytoplasmic linker proteins promote microtubule rescue in vivo." in: The Journal of cell biology, Vol. 159, Issue 4, pp. 589-99, 2002 (PubMed).

Etienne-Manneville, Hall: "Cdc42 regulates GSK-3beta and adenomatous polyposis coli to control cell polarity." in: Nature, Vol. 421, Issue 6924, pp. 753-6, 2003 (PubMed).