ANKS1B antibody (AA 107-268)

Catalog No. ABIN968022

Product Details anti-ANKS1B Antibody

Target: ANKS1B (Ankyrin Repeat and Sterile alpha Motif Domain Containing 1B (ANKS1B))

Binding Specificity: AA 107-268

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANKS1B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Cross-Reactivity: Chicken, Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EB1 aa. 107-268

Clone: 5-EB1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-ANKS1B antibody (ABIN968022)

Etienne-Manneville, Hall: "Cdc42 regulates GSK-3beta and adenomatous polyposis coli to control cell polarity." in: Nature, Vol. 421, Issue 6924, pp. 753-6, (2003) (PubMed).

Askham, Vaughan, Goodson, Morrison: "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3627-45, (2002) (PubMed).

Komarova, Akhmanova, Kojima, Galjart, Borisy: "Cytoplasmic linker proteins promote microtubule rescue in vivo." in: The Journal of cell biology, Vol. 159, Issue 4, pp. 589-99, (2002) (PubMed).

Mamane, Grandvaux, Hernandez, Sharma, Innocente, Lee, Azimi, Lin, Hiscott: "Repression of IRF-4 target genes in human T cell leukemia virus-1 infection." in: Oncogene, Vol. 21, Issue 44, pp. 6751-65, (2002) (PubMed).

Su, Huang, Fair, Powell, Heston: "Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio of expression as a potential measurement of progression." in: Cancer research, Vol. 55, Issue 7, pp. 1441-3, (1995) (PubMed).

Target Details for ANKS1B

Target: ANKS1B (Ankyrin Repeat and Sterile alpha Motif Domain Containing 1B (ANKS1B))

Alternative Name: EB1 (ANKS1B Products)

Synonyms: AIDA antibody, AIDA-1 antibody, ANKS2 antibody, EB-1 antibody, EB1 antibody, cajalin-2 antibody, AIDA-1b antibody, C030032C09Rik antibody, E530015N03Rik antibody, ENSMUSG00000078431 antibody, Gm10937 antibody, Gm1555 antibody, RGD1565556 antibody, si:ch211-146a9.3 antibody, si:dkey-11k24.3 antibody, Anks1b antibody, ankyrin repeat and sterile alpha motif domain containing 1B antibody, ankyrin repeat and sterile alpha motif domain-containing protein 1B antibody, ANKS1B antibody, Anks1b antibody, anks1b antibody, LOC100717398 antibody

Background: Familial adenomatous polyposis is an autosomal inherited disease characterized by mutations in the APC (adenomatous polyposis coli) gene. Individuals with this disease are predisposed to colorectal polyps and cancer. It has been proposed that disruption of the APC gene plays a role in the disease. APC is a large coiled-coil protein that interacts, via its carboxy terminus, with catenins and with the protein, EB1. This 30 kDa protein was discovered as a result of its strong binding to the C-terminal domain of APC. Like the APC-binding proteins beta-Catenin and pp120Cas, EB1 may play an essential role in modulating APC activity and tumorigenesis. This antibody is routinely tested by western blot analysis.

Molecular Weight: 30 kDa