BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 49-179)

Details for Product anti-BCL2 Antibody No. ABIN968024, Supplier: Log in to see
Antigen
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2-like
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • LOC100224888
  • bcl2
  • LOC100359045
  • BCL-2
Alternatives
anti-Human BCL2 antibody for Flow Cytometry
Epitope
AA 49-179
164
78
67
65
44
31
25
25
24
21
18
15
15
15
13
13
13
11
9
9
6
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
1060
257
212
54
27
22
18
18
5
4
4
3
2
2
2
1
Host
Mouse
578
547
19
6
2
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This BCL2 antibody is un-conjugated
50
50
45
38
25
24
22
16
15
14
14
14
14
14
14
14
13
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
838
398
388
341
336
326
234
195
171
81
40
15
14
13
12
11
9
4
3
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Human Bcl-2 aa. 49-179
Clone 7-Bcl
Isotype IgG1
Cross-Reactivity Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Human hematopoietic malignancies are often characterized by gene rearrangements which deregulate gene expression. The Bcl-2 gene (B-cell leukemia/lymphoma 2) was discovered by walking the chromosomal breakpoints t(14,18). This gene consists of two exons and several transcripts that encode two different protein products, Bcl-2alpha and Bcl-2beta. Both are approximately 26-30kDa and differ at their C-terminal region. Bcl-2 suppresses programmed cell death (apoptosis) and this property appears to be modulated by homodimerization or association with Bax and Bad, two cell death promoters. This antibody is routinely tested by western blot analysis.
Molecular Weight 26 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 49-179) antibody (ABIN968024) Western blot analysis of Bcl-2 (clone 7) on Jurkat lysate. Lane 1: 1:500, lane 2: 1:1...
 image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 49-179) antibody (ABIN968024) Immunohistochemical staining of Bcl2 (clone 7) on Rabbit Lung
Product cited in: Decary, Decesse, Ogryzko, Reed, Naguibneva, Harel-Bellan, Cremisi: "The retinoblastoma protein binds the promoter of the survival gene bcl-2 and regulates its transcription in epithelial cells through transcription factor AP-2." in: Molecular and cellular biology, Vol. 22, Issue 22, pp. 7877-88, 2002 (PubMed).

Naga Prasad, Laporte, Chamberlain, Caron, Barak, Rockman: "Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex." in: The Journal of cell biology, Vol. 158, Issue 3, pp. 563-75, 2002 (PubMed).

Ayllón, Cayla, García, Roncal, Fernández, Albar, Martínez, Rebollo: "Bcl-2 targets protein phosphatase 1 alpha to Bad." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7345-52, 2001 (PubMed).

Kihara, Shimohama, Sawada, Honda, Nakamizo, Shibasaki, Kume, Akaike: "alpha 7 nicotinic receptor transduces signals to phosphatidylinositol 3-kinase to block A beta-amyloid-induced neurotoxicity." in: The Journal of biological chemistry, Vol. 276, Issue 17, pp. 13541-6, 2001 (PubMed).

Background publications Tsujimoto, Croce: "Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5214-8, 1986 (PubMed).

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