Hematopoietic Cell Specific Lyn Substrate 1 (HCLS1) (AA 17-190) antibody

Antigen: Hematopoietic Cell Specific Lyn Substrate 1 (HCLS1)

Binding Site: AA 17-190

Clonality: Monoclonal (9)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968026

Additional Information

Alternative name: HS1

Immunogen: Human HS1

Format: Liquid

Isotype: IgG1

Clone: 9

Description: HS1 (lck-binding protein 1 (lckBP1)) is a protein of 486 amino acids with homology to cytoplasmic tyrosine kinases. The protein contains an SH3 domain and two proline-rich regions, that may be the binding site for lck. Co- mmunoprecipitation experiments demonstrated a tight association between HS1 and lck. The SH3 domain of lck is necessary for this interaction. In addition, HS1 contains tandem repeat sequence of seven amino acids which is a substrate for the tyrosine kinase src. This sequence is also found in the protein cortactin. Therefore, HS1, like cortactin, may be an important adaptor that is critical for the regulation of cellular tyrosine kinases. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 75 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of HS1 on human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-HS1 antibody.

Immunofluorescent staining of HL60 cells with anti-HS1 antibody.

Publications

Product: Takemoto, Furuta, Li et al.: "LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck." in: The EMBO journal, Vol. 14, Issue 14, pp. 3403-14, 1995 (PubMed).

Fukuda, Kitamura, Taniuchi et al.: "Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7302-6, 1995 (PubMed).

Yamanashi, Okada, Semba et al.: "Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3631-5, 1993 (PubMed).