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HCLS1 antibody (Hematopoietic Cell-Specific Lyn Substrate 1) (AA 17-190)

Details for Product anti-HCLS1 Antibody No. ABIN968026, Supplier: Log in to see
Antigen
  • AW213261
  • CTTNL
  • GW128
  • HCLS1
  • hcls1
  • HS1
  • KCIP-1
  • si:dkey-9a20.6
  • wu:fk96b08
  • YWHAA
  • zgc:154014
Alternatives
anti-Human HCLS1 antibody for Western Blotting
Epitope
AA 17-190
28
15
13
12
7
7
6
6
6
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
121
30
26
2
2
1
1
1
Host
Mouse
95
25
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This HCLS1 antibody is un-conjugated
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
104
57
44
20
14
11
6
2
2
1
1
1
1
1
Supplier
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Immunogen Human HS1 aa. 17-190
Clone 9-HS1
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name HS1 (HCLS1 Antibody Abstract)
Background HS1 (lck-binding protein 1 (lckBP1)) is a protein of 486 amino acids with homology to cytoplasmic tyrosine kinases. The protein contains an SH3 domain and two proline-rich regions, that may be the binding site for lck. Co- mmunoprecipitation experiments demonstrated a tight association between HS1 and lck. The SH3 domain of lck is necessary for this interaction. In addition, HS1 contains tandem repeat sequence of seven amino acids which is a substrate for the tyrosine kinase src. This sequence is also found in the protein cortactin. Therefore, HS1, like cortactin, may be an important adaptor that is critical for the regulation of cellular tyrosine kinases. This antibody is routinely tested by western blot analysis.
Molecular Weight 75 kDa
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-HCLS1 antibody (Hematopoietic Cell-Specific Lyn Substrate 1) (AA 17-190) (ABIN968026) Western blot analysis of HS1 on human endothelial cell lysate. Lane 1: 1:250, lane 2:...
Immunofluorescence (IF) image for anti-HCLS1 antibody (Hematopoietic Cell-Specific Lyn Substrate 1) (AA 17-190) (ABIN968026) Immunofluorescent staining of HL60 cells with anti-HS1 antibody.
Background publications Fukuda, Kitamura, Taniuchi, Maekawa, Benhamou, Sarthou, Watanabe: "Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7302-6, 1995 (PubMed).

Yamanashi, Okada, Semba, Yamori, Umemori, Tsunasawa, Toyoshima, Kitamura, Watanabe, Yamamoto: "Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3631-5, 1993 (PubMed).

Product cited in: Takemoto, Furuta, Li, Strong-Sparks, Hashimoto: "LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck." in: The EMBO journal, Vol. 14, Issue 14, pp. 3403-14, 1995 (PubMed).