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CaM Kinase Kinase (AA 341-504) antibody

Reactivity: Dog, Human, Rat IF, IP, IHC, WB Host: Mouse Monoclonal 6-CaM Kinase Kinase unconjugated
Catalog No. ABIN968029
  • Target
    CaM Kinase Kinase
    Binding Specificity
    AA 341-504
    Reactivity
    Dog, Human, Rat
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
    Cross-Reactivity
    Human, Dog (Canine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat CaM Kinase Kinase aa. 341-504
    Clone
    6-CaM Kinase Kinase
    Isotype
    IgG2a
  • Comment

    Related Products: ABIN968545, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Egea, Espinet, Soler, Dolcet, Yuste, Encinas, Iglesias, Rocamora, Comella: "Neuronal survival induced by neurotrophins requires calmodulin." in: The Journal of cell biology, Vol. 154, Issue 3, pp. 585-97, (2001) (PubMed).

    Wayman, Walters, Kolibaba, Soderling, Christian: "CaM kinase IV regulates lineage commitment and survival of erythroid progenitors in a non-cell-autonomous manner." in: The Journal of cell biology, Vol. 151, Issue 4, pp. 811-24, (2000) (PubMed).

    Wu, Means: "Ca(2+)/calmodulin-dependent protein kinase IV is expressed in spermatids and targeted to chromatin and the nuclear matrix." in: The Journal of biological chemistry, Vol. 275, Issue 11, pp. 7994-9, (2000) (PubMed).

    Edelman, Mitchelhill, Selbert, Anderson, Hook, Stapleton, Goldstein, Means, Kemp: "Multiple Ca(2+)-calmodulin-dependent protein kinase kinases from rat brain. Purification, regulation by Ca(2+)-calmodulin, and partial amino acid sequence." in: The Journal of biological chemistry, Vol. 271, Issue 18, pp. 10806-10, (1996) (PubMed).

    Tokumitsu, Enslen, Soderling: "Characterization of a Ca2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase." in: The Journal of biological chemistry, Vol. 270, Issue 33, pp. 19320-4, (1995) (PubMed).

  • Target
    CaM Kinase Kinase
    Background
    Ca2+/Calmodulin (CaM)-dependent kinases are multifunctional kinases involved in a myriad of cellular functions such as neurotransmitter synthesis, long-term potentiation, and formation of spatial learning. A 68kDa CaM Kinase Kinase with homology to the Ca2+/CaM-dependent kinases, phosphorylates and activates Ca2+/CaM-dependent protein kinases I and IV, but not type II. This phosphorylation occurs on Ser/Thr residues and is Ca2+ and CaM dependent. CaM Kinase Kinase is abundant in brain tissue, and is thought to be an upstream regulator in the Ca2+/CaM-dependent neural processes. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    68 kDa
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