Dual Specificity Phosphatase 3 (DUSP3) (AA 1-185) antibody

Antigen: Dual Specificity Phosphatase 3 (DUSP3)

Synonyms: VHR, T-DSP11, 2210015O03Rik, 5031436O03Rik, DUSP3, MGC189429, RGD1560049, si:bz18k17.3, si:rp71-18k17.3, MGC152292

Binding Site: AA 1-185

Clonality: Monoclonal (24)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968031

Additional Information

Alternative name: VHR

Immunogen: Human VHR

Cross-Reactivity: Dog (Canine), Rat (Rattus), Chicken

Format: Liquid

Isotype: IgG1

Clone: 24

Description: Protein tyrosine phosphatases (PTP) have been implicated in cell proliferation, cell signaling, and oncogenic transformation. More members of the PTP family of proteins have been found by screening libraries with cDNA at low stringency hybridization. VHR, a prototype for PTP, is a vaccinia-virus (VH-1)-related phosphatase of 185 amino acids with activity against phosphotyrosine and phosphoserine. In vitro, VHR is capable of dephosphorylating several growth factor receptors. In conjunction with insulin or progesterone, VHR accelerates germinal vesicle breakdown of Xenopus oocytes. These findings suggest that VHR is implicated in the regulation of cyclin-dependent kinases during the cell cycle.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 20 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of VHR on Jurkat lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of VHR.

Rabbit Liver

Publications

Product: Ishibashi, Bottaro, Chan et al.: "Expression cloning of a human dual-specificity phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 24, pp. 12170-4, 1993 (PubMed).

Aroca, Bottaro, Ishibashi et al.: "Human dual specificity phosphatase VHR activates maturation promotion factor and triggers meiotic maturation in Xenopus oocytes." in: The Journal of biological chemistry, Vol. 270, Issue 23, pp. 14229-34, 1995 (PubMed).

Yuvaniyama, Denu, Dixon et al.: "Crystal structure of the dual specificity protein phosphatase VHR." in: Science (New York, N.Y.), Vol. 272, Issue 5266, pp. 1328-31, 1996 (PubMed).

Alonso, Saxena, Williams et al.: "Inhibitory role for dual specificity phosphatase VHR in T cell antigen receptor and CD28-induced Erk and Jnk activation." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4766-71, 2001 (PubMed).