GFAP antibody (Glial Fibrillary Acidic Protein) (AA 418-432)

Details for Product anti-GFAP Antibody No. ABIN968041, Supplier: Log in to see
Antigen
  • GFAP
  • AI836096
  • cb345
  • etID36982.3
  • gfapl
  • wu:fb34h11
  • wu:fk42c12
  • xx:af506734
  • zgc:110485
  • glial fibrillary acidic protein
  • GFAP
  • LOC100136168
  • gfap
  • Gfap
Epitope
AA 418-432
69
62
32
29
27
16
15
9
6
6
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
732
394
383
197
183
137
101
38
33
32
25
24
22
21
12
7
6
4
3
3
2
2
2
1
1
1
1
1
1
1
Host
Mouse
450
314
27
23
4
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFAP antibody is un-conjugated
33
23
23
17
11
11
9
8
8
7
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
612
330
275
265
234
215
140
88
78
40
16
15
13
11
8
8
7
6
5
4
3
1
1
1
1
1
1
1
1
Options
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Immunogen Human GFAP aa. 418-432
Clone 52-GFAP
Isotype IgG1
Cross-Reactivity Chicken, Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name GFAP (GFAP Antibody Abstract)
Background The cytoskeleton is an array of highly ordered filaments that maintains the shape, structure, and functionality of cells. There are three major types of filaments: actin filaments, microtubules, and intermediate filaments. The distinctions among them are based on protein composition and arrangements. Glial fibrillary acidic protein (GFAP) is a 50 kDa component of the intermediate filaments and was originally identified in astrocytes. The overall structure of GFAP, which confirms its similarities to other intermediate filament proteins, indicates a central rod of about 130 amino acids flanked by coiled regions. GFAP has been extensively used as an immunohistochemical marker for tumors derived from astroglia. This antibody is routinely tested by western blot analysis.
Molecular Weight 50 kDa
Research Area Stem Cells, Cytoskeleton, Glia marker, Cell/Tissue Markers, Neurology
Comment

Related Products: ABIN967389, ABIN968552

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-GFAP antibody (Glial Fibrillary Acidic Protein) (AA 418-432) (ABIN968041) Western blot analysis of GFAP on SW13 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1...
Immunofluorescence (IF) image for anti-GFAP antibody (Glial Fibrillary Acidic Protein) (AA 418-432) (ABIN968041) Immunofluorescent staining of Rabbit Brain tissue section.
 image for anti-GFAP antibody (Glial Fibrillary Acidic Protein) (AA 418-432) (ABIN968041) anti-Glial Fibrillary Acidic Protein (GFAP) (AA 418-432) antibody (Image 3)
Western Blotting (WB) image for anti-GFAP antibody (Glial Fibrillary Acidic Protein) (AA 418-432) (ABIN968041) anti-Glial Fibrillary Acidic Protein (GFAP) (AA 418-432) antibody (Image 4)
Background publications Reeves, Helman, Allison, Israel: "Molecular cloning and primary structure of human glial fibrillary acidic protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 13, pp. 5178-82, 1989 (PubMed).