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Tripartite Motif Containing 25 (TRIM25) (AA 32-210) antibody

Details for Product No. ABIN968044, Supplier: Log in to see
Antigen
  • zgc:55773
  • MGC130751
  • EFP
  • RNF147
  • Z147
  • ZNF147
  • AA960166
  • AL022677
  • Zfp147
  • Efp
  • Znf147
Epitope
AA 32-210
18
15
14
14
12
11
11
5
5
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
119
31
18
1
1
1
1
1
1
Host
Mouse
107
12
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
87
50
24
20
16
15
12
4
3
2
2
Supplier
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Immunogen Human EFP aa. 32-210
Clone 2-EFP
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name EFP (TRIM25 Antibody Abstract)
Background Steriod hormones, unlike polypeptide hormones, diffuse inside the cell, bind cytoplasmic or nuclear receptors, and activate specific genes. Complete identification of such receptors would greatly increase the understanding of the celluar processes activated by steriods. EFP (Estrogen receptor Finger Protein) was identified as a result of its specific interaction with the estrogen receptor. EFP contains a zinc-finger domain, indicating that it is a DNA-binding protein. EFP transcription is regulated by estrogens and, in combination with the estrogen receptor, it may modulate the expression of estrogen-responsive genes. This antibody is routinely tested by western blot analysis.
Synonyms: Estrogen receptor Finger Protein
Molecular Weight 71 kDa
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Tripartite Motif Containing 25 (TRIM25) (AA 32-210) antibody (ABIN968044) Western blot analysis of EFP on a human endothelial cell lysate. Lane 1: 1:5000, lane...
Immunofluorescence (IF) image for anti-Tripartite Motif Containing 25 (TRIM25) (AA 32-210) antibody (ABIN968044) Immunofluorescence staining of human endothelial cells.
 image for anti-Tripartite Motif Containing 25 (TRIM25) (AA 32-210) antibody (ABIN968044) anti-Tripartite Motif Containing 25 (TRIM25) (AA 32-210) antibody (Image 3)
Product cited in: Inoue, Orimo, Hosoi et al.: "Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11117-21, 1994 (PubMed).