Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37) (AA 1-163) antibody
|Synonyms||P50CDC37, p50, p50Cdc37|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|3 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Cross-Reactivity||Dog (Canine), Rat (Rattus), Mouse (Murine)|
|Description||Progression through the cell cycle is determined by numerous extracellular factors that convey their messages to the cyclin-dependent kinases (cdk). Cdks are regulated by factors such as the availability of cyclins, inhibitors like Cip1 and Kip1, and phosphatases. CDC37 (50kDa), a regulator of the cell cycle, targets Cdk4. CDC37 was identified in a complex with pp60v-src and Raf-1. In vitro assays revealed CDC37 as a subunit of Hsp90 which associates with Cdk4. The overall result of this binding appears to be a shorter half-life of Cdk4.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||50 kDa|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Research Area||Chromatin and Nuclear Signaling, Cell Cycle, Kinases/Phosphatases|
|Restrictions||For Research Use only|
|Western blot analysis of CDC37 on human endothelial lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of CDC37. WI38 anti-Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37) (AA 1-163) antibody (Image 3)|
Stepanova, Leng, Parker et al.: "Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4." in: Genes & development, Vol. 10, Issue 12, pp. 1491-502, 1996 (PubMed).
Grammatikakis, Lin, Grammatikakis et al.: "p50(cdc37) acting in concert with Hsp90 is required for Raf-1 function." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 1661-72, 1999 (PubMed).
Lange, Rebollo, Herold et al.: "Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes." in: The EMBO journal, Vol. 21, Issue 20, pp. 5364-74, 2002 (PubMed).
|Hosts||Rabbit (37), Mouse (4)|
|Reactivities||Human (40), Mouse (Murine) (29), Rat (Rattus) (29), Cow (Bovine) (19), Dog (Canine) (19), Pig (Porcine) (18), Cat (Feline) (1), Chicken (1), Hamster (1)|
|Applications||Immunofluorescence (IF) (27), Western Blotting (WB) (25), ELISA (15), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunoprecipitation (IP) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Center (1), Internal Region (1), pSer13 (1)|