You are viewing an incomplete version of our website. Please click to reload the website as full version.

Symplekin (SYMPK) (AA 914-1080) antibody

Details for Product No. ABIN968084, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms CG2097, Dmel\\CG2097, dSymp, sympk, MGC75595, SYMPK, ENHANCED SILENCING PHENOTYPE 4, T10O8.110, T10O8_110, T22C5.3, DKFZp468C0711, SPK, SYM, Hfn3g, 1500016F02Rik, 4632415H16Rik, AA125406, AI449890
AA 914-1080
(5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(40), (11), (11), (1)
(28), (12)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(37), (14), (13), (11), (5), (4), (3), (1), (1), (1)
Pubmed 2 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 50 μg
Shipping to United States ( )
Immunogen Human Symplekin aa. 914-1080
Clone 25-Symplekin
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine), Human, Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Symplekin (SYMPK Antibody Abstract)
Background Tight junctions or zonula occludens, are specialized epithelial cell-cell contact sites that modulate the diffusion of macromolecules and ions through the intracellular space. Tight juctions are formed by the weaving of protein strands between two adjacent plasma membranes. Symplekin is a protein component of the submembraneous face of the tight junctions in polar epithelial cells, but is absent in vascular endothelium. Symplekin mRNA encodes a protein of 1142 amino acids with an apparent molecular weight of 150 kDa. Studies have shown that both symplekin mRNA and protein are present in the cytoplasm and nucleus in a variety of cells. However, Symplekin is recruited to the zonula occludens only in those cells which form functional cell-cell contacts.
Molecular Weight 150 kDa
Research Area Signaling, DNA/RNA

Related Products: ABIN968539, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Symplekin (SYMPK) (AA 914-1080) antibody (ABIN968084) Western blot analysis of Symplekin on a PC12 cell lysate (Rat neuroblastoma, ATCC CRL...
Immunofluorescence (IF) image for anti-Symplekin (SYMPK) (AA 914-1080) antibody (ABIN968084) Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).
 image for anti-Symplekin (SYMPK) (AA 914-1080) antibody (ABIN968084) anti-Symplekin (SYMPK) (AA 914-1080) antibody (Image 3)
Western Blotting (WB) image for anti-Symplekin (SYMPK) (AA 914-1080) antibody (ABIN968084) anti-Symplekin (SYMPK) (AA 914-1080) antibody (Image 4)
Product cited in: Cordenonsi, DAtri, Hammar et al.: "Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1569-82, 2000 (PubMed).

Keon, Schäfer, Kuhn et al.: "Symplekin, a novel type of tight junction plaque protein." in: The Journal of cell biology, Vol. 134, Issue 4, pp. 1003-18, 1996 (PubMed).

Catalog No. ABIN968084
Contact our Customer Service for availability and price in your country.

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?