Symplekin (SYMPK) (AA 914-1080) antibody

Antigen: Symplekin (SYMPK)

Synonyms: SPK, SYM, FLJ27092, DmelCG2097, AA125406, AI449890, 1500016F02Rik, 4632415H16Rik, HNF-3G, sympk, MGC75595, SYMPK, ENHANCED SILENCING PHENOTYPE 4, ESP4, T10O8.110, T10O8_110, T22C5.3, DKFZp468C0711

Binding Site: AA 914-1080

Clonality: Monoclonal (25)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN968084

Additional Information

Alternative name: Symplekin

Immunogen: Human Symplekin

Cross-Reactivity: Rat (Rattus), Mouse (Murine), Human, Dog (Canine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 25

Description: Tight junctions or zonula occludens, are specialized epithelial cell-cell contact sites that modulate the diffusion of macromolecules and ions through the intracellular space. Tight juctions are formed by the weaving of protein strands between two adjacent plasma membranes. Symplekin is a protein component of the submembraneous face of the tight junctions in polar epithelial cells, but is absent in vascular endothelium. Symplekin mRNA encodes a protein of 1142 amino acids with an apparent molecular weight of 150 kDa. Studies have shown that both symplekin mRNA and protein are present in the cytoplasm and nucleus in a variety of cells. However, Symplekin is recruited to the zonula occludens only in those cells which form functional cell-cell contacts.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 150 kDa

Comments:

Related Products: ABIN968539, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of Symplekin on a PC12 cell lysate (Rat neuroblastoma, ATCC CRL-1721). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Symplekin antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).

anti-Symplekin (SYMPK) (AA 914-1080) antibody (Image 3)

Publications

Product: Keon, Schäfer, Kuhn et al.: "Symplekin, a novel type of tight junction plaque protein." in: The Journal of cell biology, Vol. 134, Issue 4, pp. 1003-18, 1996 (PubMed).

Cordenonsi, DAtri, Hammar et al.: "Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1569-82, 2000 (PubMed).