Symplekin (SYMPK) (AA 914-1080) antibody

Details for Product No. ABIN968084
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Antigen
Synonyms CG2097, Dmel\\CG2097, dSymp, sympk, MGC75595, SYMPK, ENHANCED SILENCING PHENOTYPE 4, T10O8.110, T10O8_110, T22C5.3, DKFZp468C0711, SPK, SYM, Hfn3g, 1500016F02Rik, 4632415H16Rik, AA125406, AI449890
Epitope
AA 914-1080
(7), (4), (3), (2), (2), (1), (1), (1)
Reactivity
Human
(34), (7), (6), (1)
Host
Mouse
(17), (17)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(32), (15), (13), (10), (6), (5), (4), (3), (2)
Pubmed 2 references available
Quantity 50 µg
Options
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Catalog No. ABIN968084
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Immunogen Human Symplekin
Clone 25
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine), Human, Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Symplekin
Background Tight junctions or zonula occludens, are specialized epithelial cell-cell contact sites that modulate the diffusion of macromolecules and ions through the intracellular space. Tight juctions are formed by the weaving of protein strands between two adjacent plasma membranes. Symplekin is a protein component of the submembraneous face of the tight junctions in polar epithelial cells, but is absent in vascular endothelium. Symplekin mRNA encodes a protein of 1142 amino acids with an apparent molecular weight of 150 kDa. Studies have shown that both symplekin mRNA and protein are present in the cytoplasm and nucleus in a variety of cells. However, Symplekin is recruited to the zonula occludens only in those cells which form functional cell-cell contacts.
Molecular Weight 150 kDa
Research Area Signaling, DNA/RNA
Comment

Related Products: ABIN968539, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Symplekin (SYMPK) (AA 914-1080) antibody Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).
anti-Symplekin (SYMPK) (AA 914-1080) antibody (2) Western blot analysis of Symplekin on a PC12 cell lysate (Rat neuroblastoma, ATCC CRL-1721). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Symplekin antibody.
anti-Symplekin (SYMPK) (AA 914-1080) antibody (3) anti-Symplekin (SYMPK) (AA 914-1080) antibody (Image 3)
Product cited in: Keon, Schäfer, Kuhn et al.: "Symplekin, a novel type of tight junction plaque protein." in: The Journal of cell biology, Vol. 134, Issue 4, pp. 1003-18, 1996 (PubMed).

Cordenonsi, DAtri, Hammar et al.: "Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1569-82, 2000 (PubMed).

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