Death-Associated Protein 3 (DAP3) (AA 98-248) antibody

Antigen: Death-Associated Protein 3 (DAP3)

Synonyms: DAP6, EAP1, BING2, MGC126245, MGC126246, DAP-3, 4921514D13Rik, MRPS29, MRP-S29, bMRP-10, MGC126058, MGC126059, DKFZp686G12159, DAP3

Binding Site: AA 98-248

Clonality: Monoclonal (10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN968095

Additional Information

Alternative name: DAP3

Immunogen: Human DAP3

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Frog

Format: Liquid

Isotype: IgG1

Clone: 10

Description: Chronic exposure to interferons inhibits cell proliferation and results in cell death. The gene for the Death Associated Protein-3 (DAP3) was identified using a novel approach named Technical Knockout. Briefly, HeLa cells were tranfected with an antisense cDNA expression library, then exposed to interferon-gamma. Surviving cells were rescued and the anti-sense protecting genes were isolated. Several genes were identified, including one for DAP3. The DAP3 gene was found widely expressed and, as predicted, DAP3 antisense mRNA protected HeLa cells from the killing effects of interferon-gamma. Thus, this novel protein lies somewhere in the signaling downstream of interferon-gamma induced cell death.
Synonyms: Death Associated Protein-3

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 46 kDa

Comments:

Related Products: ABIN968552, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Apoptosis/Necrosis, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of DAP3 on a SW-13 cell lysate (Human adrenal gland carcinoma, ATCC CCL-105). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-DAP3 antibody.

Immunofluorescence staining of human endothelial cells.

anti-Death-Associated Protein 3 (DAP3) (AA 98-248) antibody (Image 3)

Publications

Product: Kissil, Deiss, Bayewitch et al.: "Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death." in: The Journal of biological chemistry, Vol. 270, Issue 46, pp. 27932-6, 1996 (PubMed).

Kim, Suh, Sporn et al.: "An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22320-9, 2002 (PubMed).

Fulda, Debatin: "IFNgamma sensitizes for apoptosis by upregulating caspase-8 expression through the Stat1 pathway." in: Oncogene, Vol. 21, Issue 15, pp. 2295-308, 2002 (PubMed).

Pedersen, Kitada, Schimmer et al.: "The triterpenoid CDDO induces apoptosis in refractory CLL B cells." in: Blood, Vol. 100, Issue 8, pp. 2965-72, 2002 (PubMed).