Microtubule-Associated Protein tau (MAPT) (AA 23-179) antibody

Antigen: Microtubule-Associated Protein tau (MAPT)

Synonyms: TAU, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, FTDP-17, FLJ31424, MGC138549, Tau, Mtapt, AI413597, AW045860, pTau, RNPTAU, MGC156663, tau, MGC134287, xtp, MAPT

Binding Site: AA 23-179

Clonality: Monoclonal (15)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968103

Additional Information

Alternative name: Tau

Immunogen: Human Tau

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG2b

Clone: 15

Description: In addition to tubulin, microtubules have other components named Microtubule-associated proteins (MAPs), which include the high molecular MAPs (200-400 kDa) and the Tau proteins (55-70 kDa). These proteins co-purify with the microtubules during repeated cycles of microtubule assembly and disassembly. Both MAPs and Tau proteins extend from the side of the microtubules. They form cross-bridges in neurons and bind tubulin by their C-terminal region. The heterogeneity in the molecular weight of Tau proteins is due to post-translational modifications and different numbers of tandem repeats in their C-terminal domain. Tau isoforms are selectively expressed in unique subsets of neurons and regulated during development. The highly phosphorylated Tau proteins have also been detected in the neurofibrillary tangles of Alzheimer's patients. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 50-68 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Neurology, Cytoskeleton, Alzheimer's Disease

Restrictions: For Research Use only

Images

Western blot analysis of Tau on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Tau antibody. Right:

Immunoflourescence staining of rat neurons.

anti-Microtubule-Associated Protein tau (MAPT) (AA 23-179) antibody (Image 3)

Publications

Product: Goedert, Spillantini, Potier et al.: "Cloning and sequencing of the cDNA encoding an isoform of microtubule-associated protein tau containing four tandem repeats: differential expression of tau protein mRNAs in human brain." in: The EMBO journal, Vol. 8, Issue 2, pp. 393-9, 1989 (PubMed).

Kanai, Hirokawa: "Sorting mechanisms of tau and MAP2 in neurons: suppressed axonal transit of MAP2 and locally regulated microtubule binding." in: Neuron, Vol. 14, Issue 2, pp. 421-32, 1995 (PubMed).

Alonso, Grundke-Iqbal, Iqbal: "Alzheimer's disease hyperphosphorylated tau sequesters normal tau into tangles of filaments and disassembles microtubules." in: Nature medicine, Vol. 2, Issue 7, pp. 783-7, 1996 (PubMed).

Wilson, Binder: "Free fatty acids stimulate the polymerization of tau and amyloid beta peptides. In vitro evidence for a common effector of pathogenesis in Alzheimer's disease." in: The American journal of pathology, Vol. 150, Issue 6, pp. 2181-95, 1997 (PubMed).

Takeda, Hatai, Hamazaki et al.: "Apoptosis signal-regulating kinase 1 (ASK1) induces neuronal differentiation and survival of PC12 cells." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9805-13, 2000 (PubMed).