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SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 27-172) antibody

Details for Product No. ABIN968106
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Antigen
Synonyms MADH2, madh2, fj43c06, wu:fj43c06, XSmad2, smad-2, JV18, JV18-1, MADR2, hMAD-2, hSMAD2, 7120426M23Rik, Madh2, Madr2, Smad-2, mMad2, jv18, Xmad2, madr2, hmad-2, hsmad2, jv18-1
Epitope
AA 27-172
(40), (36), (32), (21), (21), (18), (14), (13), (13), (12), (7), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(331), (188), (182), (80), (75), (65), (13), (3), (2), (1), (1), (1)
Host
Mouse
(290), (36), (12)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(12), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (2)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
(259), (150), (60), (60), (55), (50), (33), (18), (10), (6), (5), (2), (1), (1)
Pubmed 5 references available
Quantity 150 µg
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Catalog No. ABIN968106
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Immunogen Human MAD2
Clone CE6
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name hsMAD2
Background Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. The human hsMAD2 gene encodes a protein of 205 amino acids with a predicted molecular weight of 23.5 kDa. Binding of affinity purified polyclonal antibodies to the MAD2 protein prevents mitosis of HeLa cells. This indicates that, like its invertebrate relatives, MAD2 is necessary for mitosis. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2.
Synonyms: Mitotic Arrest Deficient-2
Molecular Weight 24 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 27-172) antibody Western blot analysis of MAD2 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-MAD2 antibody.
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 27-172) antibody (2) Immunofluorescence staining of rabbit spleen.
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 27-172) antibody (3) anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 27-172) antibody (Image 3)
Product cited in: Chen, Waters, Salmon et al.: "Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores." in: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 242-6, 1996 (PubMed).

Li, Benezra: "Identification of a human mitotic checkpoint gene: hsMAD2." in: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 246-8, 1996 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Iwanaga, Kasai, Kibler et al.: "Characterization of regions in hsMAD1 needed for binding hsMAD2. A polymorphic change in an hsMAD1 leucine zipper affects MAD1-MAD2 interaction and spindle checkpoint function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31005-13, 2002 (PubMed).

Babu, Jeganathan, Baker et al.: "Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation." in: The Journal of cell biology, Vol. 160, Issue 3, pp. 341-53, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968106
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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