Ikappa B alpha (AA 145-302) antibody

Antigen: Ikappa B alpha

Binding Site: AA 145-302

Clonality: Monoclonal (25)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN968113

Additional Information

Immunogen: Human IkappaBalpha

Format: Liquid

Isotype: IgG1

Clone: 25

Description: NF-kappaB is a transcription factor that regulates many cytokine and Ig genes involved in immune response, inflammation, and viral infections. NF-kappaB is induced by cytokines and lipopolysaccharide (LPS), but is inhibited by glucocorticoids. IkappaBalpha/MAD-3 inhibits the DNA-binding activity of NF-kappaB and related transcription factors. In THP-1 monocytic-like cells, LPS induces the phosphorylation and inactivation of IkappaBalpha, which results in the activation of NF-kappaB. Therefore, phosphorylation of IkappaBalpha may induce the dissociation of the IkappaBalpha/NF-kappaB complex.
Synonyms: MAD-3

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 38 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of IkappaBalpha on human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-IkappaBalpha.

Immunofluorescent staining of MCF7 cells with anti-IkappaBalpha antibody.

Publications

Product: Haskill, Beg, Tompkins et al.: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

Auphan, DiDonato, Rosette et al.: "Immunosuppression by glucocorticoids: inhibition of NF-kappa B activity through induction of I kappa B synthesis." in: Science (New York, N.Y.), Vol. 270, Issue 5234, pp. 286-90, 1995 (PubMed).

Traenckner, Pahl, Henkel et al.: "Phosphorylation of human I kappa B-alpha on serines 32 and 36 controls I kappa B-alpha proteolysis and NF-kappa B activation in response to diverse stimuli." in: The EMBO journal, Vol. 14, Issue 12, pp. 2876-83, 1995 (PubMed).

Cordle, Donald, Read et al.: "Lipopolysaccharide induces phosphorylation of MAD3 and activation of c-Rel and related NF-kappa B proteins in human monocytic THP-1 cells." in: The Journal of biological chemistry, Vol. 268, Issue 16, pp. 11803-10, 1993 (PubMed).

Nakashio, Fujita, Rokudai et al.: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." in: Cancer research, Vol. 60, Issue 18, pp. 5303-9, 2000 (PubMed).