Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 145-302) antibody

Details for Product No. ABIN968112
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms IKBA, MAD-3, NFKBI, AI462015, Nfkbi, RL/IF-1, ECI-6/IKBA
Epitope
AA 145-302
(42), (39), (31), (24), (23), (16), (8), (6), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(267), (166), (145), (41), (38), (14), (14)
Host
Mouse
(242), (32), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(205), (97), (88), (53), (40), (38), (38), (12), (8), (5), (4), (3), (3), (2), (1)
Pubmed 5 references available
Catalog no. ABIN968112
Quantity 50 µg
Price
Contact our Customer Service for availability and price in your country.
Options
Shipping to United States (Change)
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human IkappaBalpha
Clone 25
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Ikappa B alpha
Background NF-kappaB is a transcription factor that regulates many cytokine and Ig genes involved in immune response, inflammation, and viral infections. NF-kappaB is induced by cytokines and lipopolysaccharide (LPS), but is inhibited by glucocorticoids. IkappaBalpha/MAD-3 inhibits the DNA-binding activity of NF-kappaB and related transcription factors. In THP-1 monocytic-like cells, LPS induces the phosphorylation and inactivation of IkappaBalpha, which results in the activation of NF-kappaB. Therefore, phosphorylation of IkappaBalpha may induce the dissociation of the IkappaBalpha/NF-kappaB complex.
Synonyms: MAD-3
Molecular Weight 38 kDa
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 145-302) antibody Western blot analysis of IkappaBalpha on human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-IkappaBalpha.
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 145-302) antibody (2) Immunofluorescent staining of MCF7 cells with anti-IkappaBalpha antibody.
Product cited in: Haskill, Beg, Tompkins et al.: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

Auphan, DiDonato, Rosette et al.: "Immunosuppression by glucocorticoids: inhibition of NF-kappa B activity through induction of I kappa B synthesis." in: Science (New York, N.Y.), Vol. 270, Issue 5234, pp. 286-90, 1995 (PubMed).

Traenckner, Pahl, Henkel et al.: "Phosphorylation of human I kappa B-alpha on serines 32 and 36 controls I kappa B-alpha proteolysis and NF-kappa B activation in response to diverse stimuli." in: The EMBO journal, Vol. 14, Issue 12, pp. 2876-83, 1995 (PubMed).

Cordle, Donald, Read et al.: "Lipopolysaccharide induces phosphorylation of MAD3 and activation of c-Rel and related NF-kappa B proteins in human monocytic THP-1 cells." in: The Journal of biological chemistry, Vol. 268, Issue 16, pp. 11803-10, 1993 (PubMed).

Nakashio, Fujita, Rokudai et al.: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." in: Cancer research, Vol. 60, Issue 18, pp. 5303-9, 2000 (PubMed).

Hosts (242), (32), (5)
Reactivities (267), (166), (145), (41), (38), (14), (14)
Applications (205), (97), (88), (53), (40), (38), (38), (12), (8), (5), (4), (3), (3), (2), (1)
Conjugates (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Epitopes (42), (39), (31), (24), (23), (16), (8), (6), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top